ilvmНормативно-правовое регулирование в ветеринарииLegal regulation in veterinary medicine2782-6252SpbGUVM Publishing House10.52419/issn2782-6252.2026.2.234ilvm-1108Research ArticleБИОХИМИЯ, АНАТОМИЯ, ФИЗИОЛОГИЯBIOCHEMISTRY, ANATOMY, PHYSIOLOGYОпределение ветеринарных антимикробных препаратов в растительной продукции методом высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированиемDeter mination of veterinary antimicrobial drugs in plant-based products by high-performance liquid chromatography with mass spectrometric detectionhttps://orcid.org/0000-0003-2503-2888БаировА. Л.BairovA. L.

Антон Лутаевич Баиров - младший научный сотрудник химико-токсикологического отдела 

Anton L. Bairov - Junior Researcher, Chemical-Toxicology Department

abairov22@yandex.ruhttps://orcid.org/0000-0003-4706-6528КущИ. В.KushchI. V.

Ирина Вячеславовна Кущ - канд.ветеринар.наук, заведующий молодежной лабораторией 

Irina V. Kushch - Candidate of Veterinary Sciences, Head of the Youth Laboratory

https://orcid.org/0000-0003-4088-6822ОсиноваЕ. С.OsinovaE. S.

Екатерина Сергеевна Осинова - младший научный сотрудник химико-токсикологического отдела 

Ekaterina S. Osinova - Junior Researcher, Chemical-Toxicology Department

НурлыгаяноваГ. А.NurlygayanovaG. A.

Гульнара Ахметовна Нурлыгаянова - канд.ветеринар.наук, ведущий научный сотрудник отдела координации НИР

Gulnara A. Nurlygayanova - Candidate of Veterinary Sciences, Leading Researcher, Research Coordination Department

Федеральный центр охраны здоровья животныхРоссияFederal Center for Animal HealthRussian FederationВсероссийский научно-исследовательский институт ветеринарной санитарии, гигиены и экологии – филиал Федерального научного центра ВИЭВ РАНРоссияAll-Russian Research Institute of Veterinary Sanitation, Hygiene, and Ecology – Branch of the Federal Scientific Center VIEV RASRussian Federation20261408202602234242Copyright © Баиров А.Л., Кущ И.В., Осинова Е.С., Нурлыгаянова Г.А., 20262026Баиров А.Л., Кущ И.В., Осинова Е.С., Нурлыгаянова Г.А.Bairov A.L., Kushch I.V., Osinova E.S., Nurlygayanova G.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ilvm.elpub.ru/jour/article/view/1108

Антибактериальные препараты играют ключевую роль в современной научно-производственной практике, находя применение не только в лечебных и профилактических целях, но и в качестве стимуляторов роста в животноводстве и птицеводстве. Однако, данная практика, несмотря на свои преимущества, сопряжена с рядом недостатков. Широкое использование антибиотиков способствует развитию резистентности у патогенных и условно-патогенных микроорганизмов, что представляет серьезную угрозу для ветеринарии и общественного здравоохранения. Более того, применение антибактериальных средств приводит к загрязнению окружающей среды, что влечет контаминацию плодовоовощной продукции и нецелевых растений, значительно усугубляя проблему экологической безопасности. В настоящей работе представлена методика количественного определения 39 антибиотиков в продукции растениеводства методом высокоэффективной жидкостной хроматографии с массспектрометрическим детектированием (ВЭЖХ МС/МС), обеспечивающим высокую чувствительность, селективность и точность анализа. Основные валидационные характеристики методики (линейность, воспроизводимость, специфичность и предел определения) для определения ветеринарных препаратов в растительных объектах методом ВЭЖХ-МС/МС Новая методика позволяет осуществлять эффективный контроль качества и безопасности продукции растениеводства, что имеет важное значение для обеспечения продовольственной безопасности и защиты здоровья потребителей, способствуя поддержанию доверия к сельскохозяйственной отрасли и устойчивому развитию агропромышленного комплекса России.

Antibacterial agents play a key role in modern scientific and industrial practice, being used not only for therapeutic and prophylactic purposes, but also as growth promoters in animal husbandry and poultry farming. However, despite their advantages, this practice is associated with a number of drawbacks. The widespread use of antibiotics promotes the development of resistance in pathogenic and opportunistic microorganisms, posing a serious threat to veterinary medicine and public health. Moreover, the use of antibacterial agents leads to environmental contamination, resulting in the contamination of fruit and vegetable products and non‑target plants, significantly exacerbating the problem of environmental safety. This study presents a methodology for the quantitative determination of 39 antibiotics in crop production products using high‑performance liquid chromatography with tandem mass spectrometry (HPLC‑MS/MS), which ensures high sensitivity, selectivity, and accuracy of the analysis. The main validation characteristics of the methodology (linearity, reproducibility, specificity, and limit of detection) for the determination of veterinary drugs in plant materials by HPLC‑MS/MS are described. The new methodology enables effective quality and safety control of crop production products, which is essential for ensuring food security and protecting consumer health. It also contributes to maintaining public trust in the agricultural sector and supporting the sustainable development of Russia’s agro‑industrial complex.

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The authors declare that there are no conflicts of interest present.