ilvmНормативно-правовое регулирование в ветеринарииLegal regulation in veterinary medicine2782-6252SpbGUVM Publishing House10.52419/issn2782-6252.2023.4.80ilvm-669Research ArticleАКУШЕРСТВО, ГИНЕКОЛОГИЯOBSTETRICS, GYNECOLOGYПовышение жизнеспособности заморожено-оттаянных сперматозоидов петухов за счет применения ферментативных антиоксидантов в составе разбавителейIncreasing the viability of rooster sperm during cryopreservation through the use of enzymatic antioxidantsКурочкинА. А.KurochkinA. A.

Курочкин Антон Алексеевич

Anton Al. Kurochkin

ПлешановН. В.PleshanovN. V.

Плешанов Николай Вячеславович

Nikolay V. Pleshanov

Всероссийский научно-исследовательский институт генетики и разведения сельскохозяйственных животных – филиал ФГБНУ «Федеральный исследовательский центр животноводства – ВИЖ имени академика Л.К. Эрнста»РоссияAll-Russian Research Institute of Genetics and Breeding of Farm Animals - branch of the Federal State Budgetary Institution "Federal Research Center for Animal Husbandry - VIZh named after Academician L.K. Ernst"Russian Federation202324012024048084Copyright © Курочкин А.А., Плешанов Н.В., 20232023Курочкин А.А., Плешанов Н.В.Kurochkin A.A., Pleshanov N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ilvm.elpub.ru/jour/article/view/669

Криоконсервацию широко используют при создании криобанков репродуктивных клеток. В отличие от сельскохозяйственных животных, качественные показатели замороженного и оттаянного семени птиц ниже, что усложняет выбор особей для сохранения их семени. В данной работе мы изучили возможность улучшить показатели спермы петухов после замораживания и оттаивания ферментативными антиоксидантами в составе разбавителей. Известно, что при витрификации половых клеток экзогенные антиоксиданты уменьшают деструктивное влияние активных форм кислорода (АФК). Это даст возможность усовершенствовать процедуру криоконсервации, уменьшив оксидативный стресс, которому подвергаются сперматозоиды. Результаты подтвердили, что супероксиддисмутаза и каталаза в составе разбавителя ЛКС-1 для криоконсервации спермы петухов в дозах 75 МЕ/мл и 200 мкг/мл повышают жизнеспособность клеток на 3,65 % и 5,27 % соответственно. 

Cryopreservation of rooster semen has found wide application in creation of reproductive cell’s cryobanks. Compare to other farm animal’s semen, quality of frozen/thawed bird semen are often lower. This factor makes choice of individual ejaculates for the purposes of cryopreservation more difficult. In our study, we considered the possibility of improving frozen/thawed semen performance by adding enzymatic antioxidants to diluents. It has been shown that during the vitrification of reproductive cells, the addition of exogenous enzymatic antioxidants reduces the destructive effect of reactive oxygen species, which indicates the possibility of improving method by reducing oxidative stress to cells. When added to the diluent for cryopreservation rooster’s sperm LKS-1 superoxide dismutase in amount of 75 IU cell viability increased by 3,65 %, when was added catalase in amount of 200 μg/ml, cell viability increased by 5,27 %.

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The authors declare that there are no conflicts of interest present.