Distant action of acetylcholine and its toxic manifestations

Organophosphate insecticides are actively used to fight ectoparasites in cattle and sheep. These preparations are made in large quantities and are actively distributed by wind and water. Therefore, the problem of organophosphate insecticide poisoning is currently very relevant. In clinical practice, there are often circulatory disorders, the appearance in the blood of conglomerates of erythrocytes in case of POI poisoning. We conducted a series of experiments to monitor the movement of blood in the vessels of a mezoappendix of rats under anesthesia. Phosphacol in various doses was used as a poisoning agent. Blood CE activity was determined by potentiometric titration using the Radiometr autotitrator. Studying the phosphacol effect on red blood cells based on in vitro experiments. Studies have been conducted to study blood circulation in the microvessels of the mesopendicular in rats. Phosphacol was used for poisoning at a dose close to twice that of LD99 intramuscularly. A model treatment and prophylactic antidote based on atropine 5 mg / kg and carboxime 5 mg / kg was used as an antidote. Further, we studied the effect of model therapeutic and prophylactic antidote on the deformation of erythrocytes. Under the obtained results, it can be concluded that at a FOS poisoning, a decrease in blood flow velocity, and red blood cell deformation occur, numerous conglomerates are formed. All these changes deteriorate the general condition of animals in case of organophosphate poisoning. It was found that the use of model antidote, based on atropine and carboxime, helps to reduce the number of modified erythrocytes. When using an antidote for therapeutic purposes, the number of modified red blood cells is 39.3 ± 0.5 **%. The use of an antidote for prophylactic purposes reduces the number of deformed erythrocytes to 39.1 ± 0.1 **%, compared with a group of rats poisoned with phosphacol, but not receiving antidote therapy. The combined application of atropine with carboxyme contributes to the normalization of the blood flow rate to the physiological norm of 158 ± 15 rpm.

ацетилхолин, холинэстераза, летальная доза, фосфорорганические соединения, фосфорорганические инсектициды, лечебно-профилактический антидот, мускаринохолинорецепторы, acetylcholine, cholinesterase, lethal dose, organophosphate compounds, organophosphate insecticides, therapeutic and prophylactic antidote, muscarinocholinoceptors

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