Diagnosis of polycystic kidney disease in dogs
https://doi.org/10.52419/issn2782-6252.2022.2.64
Abstract
A test system has been developed for the diagnosis of polycystic kidney disease in dogs, including primers, probes, and a stub oligonucleotide for another allele. A solution of a control plasmid with a wild-type gene fragment, with a fragment of the mutant allele of the gene, or a mixture of both, imitating a heterozygote, was used as a template. The system is based on the TaqMan method. This test system allows for early diagnosis of animals with mutant alleles in order to monitor the state of health during ontogenesis, as well as exclude these dogs from breeding before their puberty.
About the Authors
Yu. V. Mukiy
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Yulia V. Mukiy, Ph.D. of Veterinary Sciences
D. I. Bogomaz
LLC "Beagle"
Russian Federation
Russian Federation
D.I. Bogomaz, Ph.D. of Biological Sciences
O. A. Pavlova
LLC "Beagle"
Russian Federation
Russian Federation
O.A. Pavlova, Ph.D. of Biological Sciences
References
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Review
For citations:
Mukiy Yu.V., Bogomaz D.I., Pavlova O.A. Diagnosis of polycystic kidney disease in dogs. Legal regulation in veterinary medicine. 2022;(2):64-66. (In Russ.) https://doi.org/10.52419/issn2782-6252.2022.2.64
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