Evaluation of the cumulative properties of the drug Tildox

Abstract

The purpose of the studies was to study the cumulative properties of the drug Tildox. Tildox is a complex antibiotic in the form of a powder. One gram of antibiotic сontains as active ingredients doxycycline hyclate - 100 mg and tylosin tartrate - 100 mg and excipients.

The assessment of the cumulative properties of the drug Tildox was carried out on laboratory white rats males of the Wistar breed weighing 220-230 g. The rats were divided into 2 groups - experimental and control. There were 10 animals in each group. In the experimental group the drug was administered intragastrically once a day in accordance with the scheme in doses from 0.1 LD₅₀ to 1.12 LD₅₀.

The scheme of drug administration was as follows. From days 1 to 4 the dose was 350 mg/kg of doxycycline hyclate and tylosin tartrate; from 5 to 8 days - 525 mg / kg; from 9 to 12 days - 770 mg / kg; from 13 to 16 days - 1190 mg / kg; from 17 to 20 days - 1750 mg / kg; from 21 to 24 days - 2625 mg / kg; from 24 to 28 days - 3920 mg / kg. Control rats were injected with 0.9% sodium chloride solution in a volume of 2.0 ml. The duration of the experiment was 28 days.

As a result of the research it was found that LD₅₀ with repeated administration of the drug was 2659±183 mg/kg of animal weight. The cumulation coefficient was 0.76. Thus repeated parenteral administration of Tildox to rats leads to its accumulation in the body which causes a cumulative effect.