Identification of clinical and biochemical markers of various forms of eclampsia in pregnant ewes

The clinical picture of the manifestation of various forms of eclampsia in pregnant ewes has been established, signs of a mild form of eclampsia in pregnant ewes are depression of the central nervous system. During the movements, the sheep staggered, the gait was uncertain. Quite often, there was a frozen pose, the animals stood with their necks outstretched and their heads held high. Characteristic signs of severe form are fibrillary twitching of the muscles of the ears, neck, and around the mouth. During the whole time, the animal was in a state of catalepsy. The sheep was lying on its side 8... 10 hours before its death, its limbs and neck were stretched out; it tried to throw its head back. Nervous excitement turned into a comatose state. Laboratory blood tests have established an increase in the level of ketone bodies above physiological limits by 2.3 times and their fractions — AcAs and HH, respectively, by 5.9 times and 1.5 times, a decrease in buffer bases to 18.41 ± 1.53 mmol/l, glucose concentration to 2.25±0.16 mmol/l, as well as the HH/AcAs coefficient to 1.53. The conducted ELISA of blood serum allowed us to obtain information about the levels of FSH, LH, etch radial values in the group of sheep with eclampsia and the control group, which significantly differed from each other (p<0.05). The content of cortisol (21.4±3.47 ng/ml), as well as progesterone (11.2± 4.31 ng/ml) remains quite significant, which does not allow for metabolic processes. In the blood of ewes with eclampsia, an increase in the concentration of ketodiene intermediates and conjugated trines was found to be 1.75 times, and the content of manol dialdehyde was 1.125±0.34 mmol/l. An integrated approach to the examination of pregnant ewes in the framework of obstetric medical examination increases the diagnostic effectiveness of eclampsia detection by 24.3% compared to traditional research methods. The diagnostic sensitivity was 75.8%, the specificity of the test was 96.2%, the prognostic value of a positive result was 94.5%, and the prognostic value of a negative result was 83.9%.>˂0.05). The content of cortisol (21.4±3.47 ng/ml), as well as progesterone (11.2± 4.31 ng/ml) remains quite significant, which does not allow for metabolic processes. In the blood of ewes with eclampsia, an increase in the concentration of ketodiene intermediates and conjugated trines was found to be 1.75 times, and the content of manol dialdehyde was 1.125±0.34 mmol/l. An integrated approach to the examination of pregnant ewes in the framework of obstetric medical examination increases the diagnostic effectiveness of eclampsia detection by 24.3% compared to traditional research methods. The diagnostic sensitivity was 75.8%, the specificity of the test was 96.2%, the prognostic value of a positive result was 94.5%, and the prognostic value of a negative result was 83.9%.

1. Avdeenko V. S. Metabolic changes in the blood of suyag sheep at the last stages of fruiting in normal and subclinical ketosis / V. S. Avdeenko, E. M. Sengaliev, A.V. Molchanov, A. N. Kozin. // Sheep, goats, wool business, 2017. - No. 4, pp.44-45.

2. Bulatov R. N. Etiology and clinical symptoms of the syndrome "OPG-Gestosis" in suyag sheep against the background of subclinical ketosis / R. N. Bulatov, V. S. Avdeenko, E. U. Baitlesov, A. K. Dnekeshev, E. M. Sengaliev // Materials of the international scientific and practical conference "Citizen of Kazakhstan – national of common future". Dedicated to the 25th anniversary of Independence of the Republic of Kazakhstan 29-30 April 2016, Uralsk, pp. 201-203.

3. Molchanov A.V. Diagnostics of various forms of the course of gestosis of suyag sheep against the background of metabolic stress / A.V. Molchanov, V. S. Avdeenko, E. M. Sengaliev // Sheep, goats, wool business. No. 3. pp. 58-60., 2018.

4. Sengaliev E. M. Metabolic stress in suyag sheep at the last stages of fruiting as a factor in the development of eclampsia / E. M. Sengaliev, V. S. Avdeenko, A.V. Molchanov // Bulletin of the Ulyanovsk State Agricultural Academy. No. 2 (42). pp. 206-209., 2018.

5. Sengaliev E.M. Metabolic parameters in pregnant sheep with subclinical ketosis / E. M. Sengaliev, V. S. Avdeenko, A. K. Kereyev // Young scientist, International Scientific Journal. 2018. - No. 10 (196), pp.46-48.

6. Chandan, K. Sen. Tocotrienols: Vitamin E beyond tocopherols / K. Sen Chandan, K. Savita, R. Sashwati // Life sciences. – 2006. – V. 78, No 18. – С. 2088 – 2098.

7. Liesegang, A. Effect of vitamin E supplementation of sheep and goats fed diets supplemented with polyunsaturated fatty acids and low in Se /A. Liesegang, T. Staub, B. Wichert, M. Wanner, M. Kreuzer // Journal of Animal Physiology and Animal Nutrition.– 2008.–No 92(3).– Р. 292–302.

8. Jacques, K.A. Selenium metabolism in animals. The relationship between dietary selenium form and physiological response / K.A. Jacques // th. Science and Technology in the Feed Industry, Proc. 17 Alltech Annual Symp.- Nottingham University Press. - 2001. - P. 319-348.

9. Surai, P.F. Is organic selenium better for animals than inorganic sources? / P.F. Surai, J.E. Dvorska // Feed Mix. - 2001. - Vol. 9. - P. 8-10.

10. Kohrle, J. Selenium Biology: facts and medical perspectives / J. Kohrle, R. Brigelius-Flohe, A. Block, R. Gartner et al. // Biol. Chem. - 2000. - Vol. 381. - P. 849-864.

11. Johannigman, J.A. Prone positioning and inhaled nitric oxide: synergistic therapies for acute respiratory distress syndrome / J.A. Johannigman, K. Jr. Davis, S.L. Miller et al. // J. Trauma. - 2001. - Vol. 50(4). - P. 589-596.