The application of the reaction of chemiluminescence for the detection of animals sensibilized by atypical microbacteria

Abstract

In the work there are given the results that testify to the possibility of using chemiluminescent (CL) study of blood serum using as a specific inducer a complex allergen of САМ to identify animals sensibilized by atypical mycobacteria. At the initial stage of the study, we were tested the 40 guinea pigs, of which we were infected the 20 animals with an atypical strain of M. intracellularae (group 1), the other (20) with M. smegmatis + M. phlei (group 2) subcutaneously at a dose of 40 mg / ml. The five intact individuals served as control. There were slaughtered for five animals of 1-2 groups on the 14th, 28th, 45th and 60th days after infection for pathoanatomical and bacteriological examination and also CL analysis of blood serum when interacting with a specific inductor of САМ. As a result of CL studies of the blood serum of animals of the 1 st and 2 nd groups there was observed the excess of the level of superweak luminescence samples of САМ-induced over spontaneous samples in 2.2 and more times from the 14th day of infection, whereas in the control group at all periods of the study the stimulation factor (SF) was significantly lower. From the biological material of guinea pigs sensitized with M. intracellularae, the original infecting culture was isolated. At the same time, in animals of the 2nd group, despite the positive result of chemiluminescence, we won’t observed the significant changes in the internal organs, the growth in sowing of biomaterial was absent. In the next stage of the studies we evaluated the effectiveness of the CL-method in a cattle-breeding farm that is free from tuberculosis, in which periodically during a planned examination there are isolated of the positive reactions to in- tradermal injection of PPD-tuberculin. As a result of the bacteriological studies along with the CL-analysis there was confirmed the persistence in the cattle’ body of atypical mycobacteria of III and IV groups on Runyon classification.