Morphological assessment of the liver under the action of a specific immunomodulator on the model of experimental tuberculosis

In this article there are present the results of analysis of the main morphometric parameters of tissue’s liver of guinea pigs infected with Mycobacterium bovis against the background of the preliminary introduction of a specific immunomodulator KIM-M2. There were selected the 15 animals for the setting of the experiment, of which we were formed the 3 groups. Five intact individuals (1st group) served as control. We injected a virulent culture of M. bovis, strain 14, subcutaneously at a dose of 0.001 mg / ml the animals of the 2nd and 3rd test groups (n = 5), while we were injected subcutaneously with KIM-M2 at a dose of 500 μg protein the animals of the 3rd group (n = 5) at 30 days before injections. We were calculated the parameters of the liver regeneration processes (parenchymal density, functional cell mass, nuclear mass, mass index of dinuclear cells, mass-mitotic index, functional karyo -cell index, average cut-off area of hepatocyte) based on the results of the morphometric measurements with calibrated ocular mesh. It was established that the introduction of Mycobacterium bovis (strain 14) by the results of morphometric examination of the liver caused the development of degenerative and destructive processes accompanied by plethora, stagnation and cholestasis, as well as a decrease in the depth of reparative reserves and the intensity of hepatocyte functioning. The introduction of a specific immunomodulator KIM-M2 at 30 days before infection with mycobacteria of animals contributed to a significant decrease in the severity of degenerative and destructive processes, a decrease in plethora, stagnation and bile retention in the biliary tract, and an increase in the volume of reparative liver reserves. The obtained data of a morphological evaluation of the liver state indicated an increase in the resistance of laboratory animals to infection with pathogenic mycobacteria.

микобактерия, туберкулез, специфический иммуномодулятор КИМ-М2, антиген-полимерный комплекс, mycobacterium, tuberculosis, specific immunomodulator KIM-M2, antigen-polymer complex

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