The efficacy of various protocols of colloidal centrifugation of bovine sperm for IVF
https://doi.org/10.52419/issn2782-6252.2023.3.63
Abstract
The article presents data on the effectiveness of using various sperm preparation protocols by colloidal centrifugation (density gradient centrifugation method) before procedures of assisted reproductive technologies, including in vitro artificial insemination. The use of the method can affect the quality of sexual gametes and the viability of embryos, increase the percentage of successful embryotransplantation and contribute to the appearance of highly productive offspring.
As a result of the research, the most optimal protocol of centrifugation by the method of gradient layering with indicators of the first and second centrifugation 700g – 20 min and 100g – 10 min, respectively, was determined by morphofunctional characteristics. The average rate of motile spermatozoa was 84.1±5.1%, progressive spermatozoa – 68.7±5.1%, the rate of rectilinear translational motion was 49.69 microns/sec. Morphology and viability meet quality standards.
About the Authors
G. S. Nikitin
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
PhD of Veterinary Sciences, Docent
V. V. Achilov
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
PhD of Veterinary Sciences, Docent
I. I. Mirzakaeva
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Bachelor of Biology
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Review
For citations:
Nikitin G.S., Achilov V.V., Mirzakaeva I.I. The efficacy of various protocols of colloidal centrifugation of bovine sperm for IVF. Legal regulation in veterinary medicine. 2023;(3):63-66. (In Russ.) https://doi.org/10.52419/issn2782-6252.2023.3.63
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