Isolation of cultures of microorganisms from pathological material from cows

Abstract

Rapidly developing antibiotic resistance limits the capabilities of veterinary specialists and doctors in the fight against infectious diseases. Effective diagnosis and continuous improvement in the field of antimicrobial therapy is the main approach in the treatment and prevention of bacterial infections. According to available data, bacteria are able to develop resistance in 2-3 years, while it takes an average of 5 years to develop and test a new antimicrobial drug. In our study, bacteriological examination of samples of pathological material from cows from 10 livestock farms in different districts of the Leningrad region was carried out, followed by determination of the sensitivity of isolated crops to antibiotics. In this study, 84 samples from cows were selected and examined and 115 cultures of microorganisms were isolated. As a result of the bacteriological study of the pathological material from cows, 9 groups and types of pathogenic and opportunistic microorganisms were identified. The main proportion is detected on staphylococci – 35.7%, mainly Staphylococcus aureus, epidermal staphylococcus and citrus staphylococcus. Escherichia coli (23.5%) and Proteus vulgaris (20.9%) were among the microorganisms in terms of frequency of occurrence. Most of the isolated cultures had average sensitivity to gentamicin, enrofloxacin, ciprofloxacin, ceftriaxone, cefepim, cefotaxime, neomecin; resistant to doxycycline, amoxicillin, levofloxacin, colistin, doxycycline, streptomycin, tylosin, tetracycline.