The mechanical strength of the epithelial layer of the intestine of carp after exposure to cupric acetate

Abstract

The article is devoted to the evaluation of the toxic effects of copper acetate on the body of fish. The aim of the study was to study the mechanical strength of the epithelial layer of the carp intestine after exposure to copper acetate. With the normal functioning of the gastrointestinal tract of fish, the exalted, dead, epithelial cells of the intestine are exfoliated, which is the norm. The presence of "living" epithelial cells in intestinal preparations indicates a violation of the mechanical strength of the epithelial layer of the intestine, which, in turn, is a sign of poisoning. The experiment used 3 groups of fish (ordinary carp) in 10 individuals: control group; group contained in a solution of copper acetate (1 mg/l); group contained in a solution of copper acetate (10 mg/l). Exposure to toxic solution was 4 hours. Made smear-imprint of the intestine of the carp was carried out exfoliating counting live cells. The study showed that when exposed to copper acetate with a concentration of 1 mg/l, the number of "living" cells that retained tinctorial properties is 35.28% of the total number of fused cells, and after exposure to copper acetate with a concentration of 10 mg/l - 67.85% of the total number of exfoliating cells. There is a decrease in the mechanical strength of the epithelial layer of the intestine, manifested in an increase in the desquamation (exfoliation) of living epithelial cells due to the action of a toxicant.