Development of a molecular biological method for diagnosing hemorrhagic enteritis of turkeys

Abstract

Due to the intensive development of industrial poultry farming, timely monitoring of infectious diseases of birds is an integral part of a set of measures that provides for the introduction into practice of diagnostic studies that meet the current level of scientific development.

The most relevant for industrial turkey breeding is the monitoring of particularly dangerous diseases (OIE list): Newcastle disease (ND), avian influenza (AI) and economically significant diseases of turkeys - hemorrhagic enteritis of turkeys (HET), infectious encephalomyelitis of birds (IEB), respiratory mycoplasmosis and mycoplasmic synovitis caused by Mycoplasma gallisepticum (MG) and Mycoplasma meleagridis (MM).

An analysis of the current state of research on this topic has shown that the development of diagnostic methods for immunosuppressive diseases of birds (hemorrhagic enteritis of turkeys) is an urgent area in the Russian Federation [1]. In order to prevent an outbreak of this disease, which is from an economic and epizootological point of view one of the most dangerous for the turkey breeding industry, it is necessary to carry out diagnostic monitoring of livestock on a planned basis in order to monitor the well-being of the farm for this disease, and in case of early detection of hemorrhagic enteritis, take all necessary measures to prevent further spread and reduce damage which she could have inflicted.

That is why the purpose of our study is to develop a molecular biological method for diagnosing HET using the example of PCR in the classical version.

In the course of the work carried out, the least variable GEI protein was selected, as well as the correct primers, amplification stages and the composition of the PCR mixture for this protein. When setting up the reaction, a positive result was obtained. All delivered samples reacted in accordance with the expected results.