Comparative analysis of the cryopreservation protocols efficiency of goat sperm (review)
https://doi.org/10.52419/issn2782-6252.2024.4.57
Abstract
The aim of this work is to analyze scientific and practical data on protocols for cryopreservation of sperm of breeding goats and the effectiveness of their use. Numerous studies have established that seasonality is a characteristic reproductive function, with the greatest sexual activity observed in the autumn-winter period [1]. Goat sperm has the following species-specific features: sperm membranes contain a high level of unsaturated fatty acids, while the cytoplasm of cells, on the contrary, contains an insignificant amount of antioxidant compounds. In connection with these spermatozoa, especially strong lipid peroxidation induced by free radicals (hydroxyl radicals, UK peroxide), which cause structural damage to sperm membranes during freezing-thawing or storage in a cold state at 2-4 ° C [25, 26]. Taking these facts into account, an effective cryopreservation protocol, according to the results of numerous studies, is additional filtration of sperm for effective removal of accessory sex gland secretion, use of a diluent without egg yolk, cooling at 4-5 °C for 1.5-4 hours, packing in 0.25 - 0.5 ml straws. When using 0.5 ml straws, freezing is carried out in two stages: in liquid nitrogen vapor at a height of 4 cm for 5 minutes, then further immersion in liquid nitrogen. When using 0.25 ml straws, freezing is carried out in three stages: perforated glasses are placed 16 cm above the liquid nitrogen level for 2 minutes, then 4 cm for 3 minutes, after which they are completely immersed in liquid nitrogen for long-term storage.
About the Authors
D. E. Glavatskaya
Saint Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Daria Ev. Glavatskaya, PhD student
E. A. Korochkina
Saint Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Elena Al. Korochkina, Dr.Habil. of Veterinary Sciences, Docent
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Review
For citations:
Glavatskaya D.E., Korochkina E.A. Comparative analysis of the cryopreservation protocols efficiency of goat sperm (review). Legal regulation in veterinary medicine. 2024;(4):57-62. (In Russ.) https://doi.org/10.52419/issn2782-6252.2024.4.57
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