Peculiarities of the isolation of bacteriophages specific to Klebsiella pneumoniae from wastewater of livestock complexes

Abstract

In connection with the growing resistance of infectious agents to antibacterial drugs included in the scheme of therapeutic measures of livestock complexes and the lack of new antibiotic options, phagotherapy is attracting more and more attention. Infectious diseases associated with «ESKAPE» (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.) are a global problem. Detection of phages to these pathogens is the most important step in the development of phage therapy for its introduction into the complex of anti-epizootic measures. Wastewater contains a highly diverse population of bacteria and associated bacteriophages and is therefore an attractive source of bacteriophages against bacterial pathogens associated with infectious diseases of cattle. Since wastewater is a highly contaminated material, its sample preparation represents an important step in the isolation of bacteriophages. This work presents an efficient protocol for the preparation of wastewater for the isolation of phages specific to Klebsiella pneumoniae associated with bovine bronchopneumonia. The material (n=25) was subjected to multiple filtrations using membrane filters with pore diameters of 0.45 µm and 0.22 µm and further using the accumulation method.