Study of subchronic toxicity of the drug " Prazicide-complex" on rats

Abstract

The drug «Prasicide-complex» belongs to the combined antiparasitic drugs with a wide spectrum of action.
The study of subchronic toxicity of the drug allows us to assess the safety of its use in animals. Monitoring the blood parameters of laboratory animals, namely the study of hematological and biochemical values, is one of the most important means of assessing the health of rats.
The purpose of our research was to study the blood parameters of laboratory rats when studying the subchronic toxicity of the drug «Prasicide-complex». Subchronic toxicity was studied on white Wistar laboratory rats (n = 32) of both sexes weighing 210 - 230 g. For the study, the animals were divided into 3 experimental and 1 control group, each group included 4 males and 4 females, selected according to the principle of analogues.
Animals of the first group received the drug cutaneously at a dose of 1/5 of the LD50 (100 mg/kg) daily for 21 days. The second group received the drug at a dose of 1/10 of the LD50 (50 mg/kg). The third group received a three-time therapeutic dose (750 mg/kg). The fourth group was the control (intact) [2].
Based on the data obtained during toxicological experiments, it can be concluded that the use of the drug «Prasicide-complex» in dosages of 1/5 of LD50 (100 mg/kg), 1/10 of LD50 (50 mg/kg), as well as in a triple therapeutic dose (750 mg/kg), with long-term (21 days) cutaneous exposure does not provoke the development of toxic effects in experimental subjects rats.
The results of the analysis of hematological and biochemical parameters of the blood of experimental animals indicate that the use of the studied drug «Prasicide-complex» in the indicated dosages does not cause significant deviations, which indicates the absence of a negative effect on the functions of the liver and kidneysand on the hematopoietic system.