Evaluation of the influence of combined disinfectants of the QAC group on the microstructure of rat organs

The use of disinfectants, including those based on quaternary ammonium compounds (QACs), for treating livestock buildings, especially in the presence of animals and birds, may adversely affect biological structures. The aim of our research was to determine the effect of disinfectants containing QACs on the microstructure of the liver, spleen, kidneys and small intestine of laboratory animals. The objects of the research were Wistar rats weighing 350 to 400 grams, which were divided into four groups of 12 animals according to the pairanalogue principle. The animals were administered orally 0.2% solutions of the following disinfectants in working dilutions: Group 1 - KEMICID PLUS; Group 2 - K-DEZ AIR; Group 3 - KEMISEPT and Group 4 served as a control. On the 14th day, animals were slaughtered in accordance with GOST 33216-2014 and the Requirements for Handling Laboratory Animals (2007). Histological preparations were made using the generally accepted method. The study of liver micropreparations of animals in the experimental groups showed no changes in the parenchyma and stroma cells. Active processes of physiological regeneration of the organ parenchyma were observed in animals in all experimental groups. In the spleen, in the white pulp, reproduction centers without significant proliferative activity are observed, and, in most cases, the mantle and marginal zones are well differentiated. In the renal cortex, slight perivascular edema and cellular infiltration with lymphocytes are observed in the area of the vascular pole of the renal corpuscle. The results of studies of histological sections stained with hematoxylin and eosin showed that the use of disinfectants based on substances of the quaternary ammonium compound group does not have a significant effect on the microstructure of the internal organs of rats, which indirectly proves the absence of a cytotoxic effect on the organs and systems studied.

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