Comparative assessment of diagnostic methods for genital mycoplasmosis in cattle
https://doi.org/10.52419/issn2782-6252.2025.2.45
Abstract
The issues of accurate and timely diagnosis of animal diseases, as well as improvement of their methods, have always been and remain relevant. They become especially important when the disease is characterized by a nonspecific clinical picture and the presence of a long latency period, which is observed in genital mycoplasmosis of cattle. The literature review examines the most common methods of laboratory diagnosis of this disease, noting their advantages and disadvantages regarding their use in industrial animal husbandry. Based on the conducted comparative analysis, the use of one or another diagnostic method is justified, based on the goals and objectives of the study.
About the Author
R. M. Vasiliev
Saint Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Roman M. Vasiliev - Cand. of Veterinary Science, Docent
References
1. Borkhsenius S.N., Chernova O.A., Chernov V.M., Vishnyakov I.E. Mycoplasmas in biology and medicine of the early 21st century. St. Petersburg: Nauka, 2016. 333 p. (In Russ)
2. Hazelton M.S., Morton J.M., Bosward K.L., et al. Mycoplasma species in vaginas of dairy cows before and after exposure to bulls and their association with conception. Journal of Dairy Science. 2020;103(12): 11795-11805. doi.org/10.3168/jds.2020-18758
3. Alhussen M.A., Kirpichenko V.V., Yatsentyuk S.P. et al. Pathogenic mycoplasmas of cattle Mycoplasma bovis, M. bovigenitalium and M. dispar: brief characteristics of the pathogens (review). Agricultural biology. 2021;56(2): 245-260 (In Russ)
4. Langford E.V. Mycoplasma species recovered from the reproductive tracts of western Canadian cows. Can J Comp Med. 1975; 39(2): 133-138
5. Vasiliev R.M., Vasilieva S.V. Immuno-biological properties of vaginal discharge in healthy and mycoplasmosis-infected cows. Medical Immunology (Russia). 2021; 23(4): 987-990. (In Russ) DOI 10.15789/1563-0625-IBP-2278
6. Alhussen M.A., Nesterov A.A., Kirpichenko V.V. et al. Spread of bovine mycoplasmosis on livestock farms in the Russian Federation in the period from 2015 to 2018. Veterinary Science Today. June. 2020; 2 (33): 102-108 (In Russ)
7. Vasiliev R.M. The role of chlamydial and mycoplasma infection in the etiology of infertility in cattle. International Bulletin of Veterinary Science. 2008;3: 15-16 (In Russ)
8. Ghanem M.E., Higuchi H., Tezuka E. et al. Mycoplasma infection in the uterus of early postpartum dairy cows and its relation to dystocia and endometritis. Theriogenology. 2013;79(1):180 - 185
9. Pohjanvirta T., Vähänikkilä N., Mutikainen M. et al. Transmission of Mycoplasma bovis infection in bovine in vitro embryo production. Theriogenology. 2023;199: 43-49, doi.org/10.1016/j.theriogenology.2023.01.011
10. Krasikov A.P., Rudakov N.V. Mycoplasmosis of humans and animals, their epidemiological and epizootological significance. Omsk: OOO IC "Omsk Scientific Bulletin". 2015. 717 p. (In Russ)
11. Parker A.M., Sheehy P.A., Hazelton M.S. et al. A review of mycoplasma diagnostics in cattle. J. Veterinary Internal Medicine. 2018;3 (32): 1241-1252
12. McVey D.S., Kennedy M.A., Chengappa M.M. Veterinary Microbiology. 3rd ed. Wiley-Blackwell. 2013. 629 p.
13. Dudek K., Nicholas R.A.J., Szacawa E., Bednarek D. Mycoplasma bovis Infections—Occurrence, Diagnosis and Control. Pathogens. 2020; 8(9): 1-21.
14. Babacan O., Acan M., Öncel T. et al. Diagnosis of Mycoplasma bovis infection in cattle by ELISA and PCR. Kafkas Universitesi Veteriner Fakultesi Dergisi. 2014;2(20):249-252.
15. Hazelton M.S., Morton J.M., Bosward K.L. et al. Isolation of Mycoplasma spp. and serological responses in bulls prior to and following their introduction into Mycoplasma bovis-infected dairy herds. Journal of Dairy Science. 2018;101(8): 7412-7424. doi.org/10.3168/jds.2018-14457
16. Wawegama N.K., Browning G.F., Kanci A. et al. Development of a recombinant protein-based enzymelinked immunosorbent assay for diagnosis of Mycoplasma bovis infection in cattle. Clinical and Vaccine Immunology: CVI. 2014;2 (21): 196-202.
17. Sun Z., Fu P., Wei K. et al. Identification of novel immunogenic proteins from Mycoplasma bovis and establishment of an indirect ELISA based on recombinant E1 beta subunit of the pyruvate dehydrogenase complex. PLoS ONE. 2014; 2(9): 8 p.
18. Appelt S., Aly S.S., Tonooka K. et al. Development and comparison of loop-mediated isothermal amplification and quantitative polymerase chain reaction assays for the detection of Mycoplasma bovis in milk. Journal of Dairy Science. 2019;102(3):1985-1996. doi.org/10.3168/jds.2018-15306
19. Wisselink H.J., Smid B., Plater J. et al. A European interlaboratory trial to evaluate the performance of different PCR methods for Mycoplasma bovis diagnosis. BMC Veterinary Research. 2019;1(15): 86.
20. Neder V.E., Amadio A.F., Calvinho L.F. Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina. Revista Argentina de Microbiología. 2022;Vol. 54, Issue 2.: 158-161. doi.org/10.1016/j.ram.2021.07.001
21. O’Neill R., Mooney J., Connaghan E. et al. Patterns of detection of respiratory viruses in nasal swabs from calves in Ireland: A retrospective study. Veterinary Record. 2014;14 (175): 351-351
22. Edwards D.J., Holt K.E. Beginner’s guide to comparative bacterial genome analysis using next-generation sequence data. Microbial Informatics and Experimentation. 2013;1(3): 1-9.
23. Hata E., Nagai K., Murakami K. Complete genome sequence of Mycoplasma bovigenitalium Strain HAZ 596 from a bovine vagina in Japan. Genome Announcements. 2017;6(5): 8 p.
Review
For citations:
Vasiliev R.M. Comparative assessment of diagnostic methods for genital mycoplasmosis in cattle. Legal regulation in veterinary medicine. 2025;(2):45-49. (In Russ.) https://doi.org/10.52419/issn2782-6252.2025.2.45
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