Determination of subchronic cutaneous toxicity of a new veterinary drug based on octenidine dihydrochloride

Abstract

Subchronic dermal toxicity is a characteristic that shows the negative effect of repeated repeated application of the drug for 21-28 days. The study was conducted in accordance with GOST 33216-2014 "Guidelines for the maintenance and care of laboratory animals", GOST 32642-2014 "Testing methods for the effects of chemical products on the human body. Determination of toxicity by repeated/repeated skin intake 28/21-day test". For the study, Wistar rats weighing 190-200 g were used. Control and study groups were formed, each with 10 animals. In the studied group of animals, a new medicinal product developed by us for veterinary use containing 0.1% octenidine dihydrochloride and 5% pantothenic acid was applied to a shaved skin area occupying 10% of the body area. The drug was applied to a sterile gauze cloth and attached to the skin surface for 6 hours. The study was conducted daily for 28 days. A saline solution was applied to the control group in the same way. The condition of the animals was assessed: the quality of the coat and skin, the condition of visible mucous membranes, body temperature, the state of the gastrointestinal, respiratory, nervous, and urinary systems. The animals were weighed weekly. According to the results of the experiment, there were no deaths of animals, hematological and biochemical blood parameters were unchanged, the body weight of the animals was normal during the experiment, and no changes were detected during the pathological autopsy of the animals.