Analysis and investigation of the need to develop measures for the prevention of viral diarrhea in cattle in farms of the Leningrad Region

Viral diarrhea in cattle is a contagious disease of predominantly young animals, which in the last 5 years has become widespread in the world and in Russia. The purpose of the work is to identify the need for developing a scheme for the specific prevention of viral diarrhea in cattle in the Leningrad Region on the basis of epizootic, serological, and molecular-biological studies. For the serological study of the livestock, a "Set of erythrocyte diagnosticum for serodiagnosis of viral diarrhea of cattle in the reaction of indirect haemagglutination" was used (Agrovet, Russia). PCR was carried out with sets of "VD", "MIC-COM", "HLA-COM" (Russia). Reverse transcription and real-time amplification were performed in a Rotor-Gene instrument (Corbett Research, Australia). PCR results for electrophoretic detection of amplification products were taken into account using the Gel Imager video system and the Gel Analyzer software. An analysis of the dynamics of livestock in the Leningrad Region over the past 10 years has been carried out. The results of serological screening and molecular-genetic identification of the causative agent of viral diarrhea in cattle in the conditions of the Leningrad Region are presented. The absence of a common scheme for diagnosis, prevention and treatment of the disease has been identified, which necessitates the development of a scheme for specific prevention of cattle viral diarrhea and a system for diagnostic monitoring of the disease in the Leningrad Region.

вирусная диарея, полимеразно-цепная реакция, серологические исследования, крупный рогатый скот, viral diarrhea, polymerase chain reaction, serological tests, cattle

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