Detremination of free ammonia in samples of rainbow trout (Onchorynchys mykiss) using «SMARTBIOTIC»
https://doi.org/10.52419/issn2782-6252.2022.2.122
Abstract
Fish is a product that, under improper storage and transportation conditions, can quickly undergo putrefactive spoilage, therefore, one of the indicators of fish spoilage is the detection of free ammonia in fish samples. The determination of ammonia formed during fish spoilage is based on the fact that it forms a white cloud of ammonium chloride in the presence of hydrochloric acid. According to the intensity, the rate of cloud formation and its stability, the degree of spoilage of fish is judged. the purpose of our study was to determine the effect of the use of the drug "SmartBiotic", produced on the basis of humic acids, on the quality and safety of the fish products obtained, namely, the detection of free ammonia in the selected samples of rainbow trout.
Three groups of rainbow trout fingerlings (Oncorhynchus mykiss) were formed to conduct the study. One group served as a control, and the other two were experimental subjects. The first experimental group of fingerlings received the drug at a concentration of 0.15%, and the second experimental group – at a dose of 0.25%. In the control group of rainbow trout, the drug "SmartBiotic" was not used.
About the Authors
L. Yu. KarpenkoRussian Federation
Larisa Yu. Karpenko, Dr.habil of Biological Sciences, Professor
A. A. Bakhta
Russian Federation
Alesia A. Bakhta, Ph.D. of Biological Sciences, Docent
K. P. Ivanova
Russian Federation
Katerina P. Ivanova
P. A. Polistovskaya
Russian Federation
Polina A. Polistovskaya
D. A. Orlova
Russian Federation
Diana A. Orlova, Ph.D. of Veterinary Sciences, Docent
T. V. Kalyuzhnaya
Russian Federation
Tamara V. Kalyuzhnaya, Ph.D. of Veterinary Sciences, Docent
References
1. Analysis of indicators of lysozyme activity of blood serum of rainbow trout (Oncorhynchus mykiss) when using the drug "Smartbiotic" / L. Yu. Karpenko, A. A. Bakhta, K. P. Ivanova [et al.] // Questions of legal regulation in veterinary medicine. - 2021. - No. 4. - P. 140-142. – DOI 10.52419/issn2072-6023.2021.4.140.
2. Glaskovich M.A., Karpenko L.Yu., Bakhta A.A., Kinarevskaya K.P. Influence of the VitoLAD biocorrector on the veterinary and sanitary indicators of broiler chicken meat // International Veterinary Bulletin. - 2018. - No. 4. - S. 78-84. – DOI 10.17238/issn2072-2419.2018.4.78.
3. Talybov R.Kh., Vasiliev R.O., Yugatova N.Yu., Gaponova V.N. Influence of the drug "tulimkar" on the bactericidal activity of blood and phagocytic activity of rat leukocytes on the background of X-ray irradiation // Agrarian science - agricultural production: materials of the International Scientific and Practical Conference: in 3 volumes, Izhevsk, February 12–15, 2019 / Ministry of Agriculture of the Russian Federation, Izhevsk State Agricultural Academy. - Izhevsk: Izhevsk State Agricultural Academy, 2019. - P. 130-133.
4. Karpenko L. Yu., Bakhta A. A., Ivanova K. P. [et al.] Studying the effect of using the biologically active water complex "HALPI" on the immunological status of elderly dogs // Questions of legal regulation in veterinary medicine . - 2020. - No. 2. - S. 102-105. – DOI 10.17238/issn2072-6023.2020.2.102.
5. Kovalev, S.P. Dynamics of some humoral indicators of innate immunity in calves with enteritis / S.P. Kovalev, V.A. Trushkin // Scientific notes of the Kazan State Academy of Veterinary Medicine. N.E. Bauman. - 2015. - T. 221. - S. 118-121.
6. Glaskovich M. A., Karpenko L. Yu., Bakhta A. A., Kinarevskaya K. P. Evaluation of the influence of the use of various biologically active additives in the diet of birds on the physicochemical parameters of meat // International Veterinary Bulletin. - 2018. - No. 2. - P. 54-59.
7. Pogodaeva, P. S. Influence of various thermostable antigens on the formation of local immunity of the mammary gland / P. S. Pogodaeva, L. Yu. Karpenko, V. S. Ponamarev // International Veterinary Bulletin. - 2021. - No. 1. - S. 247-251. – DOI 10.17238/issn2072-2419.2021.1.247.
8. Trushkin V.A., Kovalev S.P., Voinova A.A. [et al.] Results of the use of the probiotic Vetom 1.1 in enteritis in calves // Modern problems of veterinary pathology and biotechnology in the agro-industrial complex: materials of the International scientific-practical conference dedicated to the 95th anniversary of the RUE "Institute of Experimental Veterinary Medicine named after S.N. Vyshelessky", Minsk, November 16–17, 2017 / Institute of Experimental Veterinary Medicine named after S.N. S.N. Vyshelessky. - Minsk: Belarusian Science, 2017. - S. 275-278.
9. Trushkin, V. A. The use of the probiotic "Avena" for the prevention of enteritis in calves / V. A. Trushkin, I. V. Nikishina, O. F. Shumakov // Issues of legal regulation in veterinary medicine. - 2014. - No. 3. - P. 256-258.
10. Firsova, V. E., Bokhan P. D., Karpenko L. Yu. Influence of the drug "Multibacterin" on the biochemical parameters of blood and factors of innate immunity in broiler chickens // Issues of legal regulation in veterinary medicine. - 2019. - No. 1. - S. 299-302. – DOI 10.17238/issn2072-6023.2019.1.299.
Review
For citations:
Karpenko L.Yu., Bakhta A.A., Ivanova K.P., Polistovskaya P.A., Orlova D.A., Kalyuzhnaya T.V. Detremination of free ammonia in samples of rainbow trout (Onchorynchys mykiss) using «SMARTBIOTIC». Legal regulation in veterinary medicine. 2022;(2):122-125. (In Russ.) https://doi.org/10.52419/issn2782-6252.2022.2.122