Analysis of the efficiency of a rapid test for the early diagnosis of mastitis in cows
https://doi.org/10.52419/issn2782-6252.2022.4.127
Abstract
This article describes the results of a study of the content of somatic cells in milk using various methods. Milk samples were tested using a standardized apparatus method using a Somatos-V viscometer, from which 3 groups of 20 samples were selected. The formation of groups was based on the content of somatic cells in the samples (up to 200; 200-400 and over 400 thousand/cm3). Further research was aimed at studying the diagnostic properties of the new test, the action of which is based on the suppression of foaming by mastitis milk. All samples were tested using the foam test and the California mastitis test. The coincidence of the results of the study of the studied test systems with the reference method was revealed in relation to samples containing a small (up to 200 thousand/cm3) and an increased (over 400 thousand/cm3) number of somatic cells. In samples with a cell concentration from 200 to 400 thousand/cm3, there are different results in the study by express testing methods. The foam test showed 90% and the California test showed 75% agreement with the results of viscometry. Given the difficulty in diagnosing latent mastitis, the foam test studied in this work may have promising prospects for use on farms for the early detection of sick cows. This method allows more accurately, qualitatively, to determine the increase in the number of somatic cells in milk, which is important in the diagnosis of latent subclinical mastitis and their timely treatment.
Keywords
cows,
milk,
mammary gland,
mastitis,
somatic cells,
rapid test,
lauryl sulfate,
viscometry,
sulfanol
About the Authors
N. A. Panova
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Ph.D. of Biological Sciences, Docent
S. V. Vasilyeva
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Ph.D. of Veterinary Sciences, Docent
References
1. Skopichev, V. G. Immunobiology of the mammary gland and milk productivity / V. G. Skopichev, V. B. Prozorovsky. - Beau Bassin: Lap LAMBERT Academic Publishing, 2018. - 328 p.
2. Skopichev, V. G. Milk: textbook. allowance / V. G. Skopichev, N. N. Maksimyuk. - St. Petersburg: Prospekt Nauki, 2011. - 368 p.
3. Sviridenko, G.M. Mastitis in cattle / G.M. Sviridenko, E.G. Semenova // Dairy industry. - 2003. - No. 10. - S. 37-42.
4. Samburov, N.V. Improving the biological properties of colostrum / N.V. Samburov // Bulletin of the Kursk State Agricultural Academy. - 2008. - No. 2. - P. 28-29.
5. Matricardi P. M., Bockelbrink A., Beyer K., Keil T., Niggemann B., Gruber C., Wahn U., Lau S. Primary versus secondary immunoglobulin E sensitization to soy and wheat in the Multi-Centre Allergy Study cohort // Clin Exp Allergy. 2008: 38: R. 493–500.
6. Miyazawa T., Itahashi K., Imai T. Management of neonatal cow’s milk allergy in high-risk neonates // Pediatr Int. 2009; 51: R.544-547.
7. Interstate standard. GOST 23453-14. Raw milk. Methods for determining somatic cells. Standartinform. - M. - 2015. - 16 p.
8. Technical regulation of the Customs Union TR CU 033/2013 "On the safety of milk and dairy products" // Electronic fund of legal and regulatory documents [Electronic resource] - URL: https://docs.cntd.ru/document/499050562?marker= 1V9E9JF§ion=text - (Accessed 11/15/2022).
9. Kozeev E. V., Terleev S. Yu., Shkil N. A. [et al.] Peculiarities of the IKM-3 device and results of express diagnostics of abnormal cow milk // Scientific and technical progress in agricultural production. Agrarian science - agricultural production in Siberia, Kazakhstan, Mongolia, Belarus and Bulgaria: Proceedings of the International Scientific and Technical Conference. In 2 volumes, Minsk, October 19–21, 2016. - Minsk: Republican Unitary Enterprise "Scientific and Practical Center of the National Academy of Sciences of Belarus for Agricultural Mechanization", 2016. - P. 64-69. – EDN YNSAGD.
10. Shabshaevich, M. L. Determination of the content of somatic cells in raw milk / M. L. Shabshaevich, V. P. Shidlovskaya // Dairy industry. - 2007. - No. 2. - S. 30-32. – EDN HYLTJJ.
11. Patent No. 2444999 C1 Russian Federation, IPC A61B 10/00. Method for diagnosing early hypogalactia: No. 2010126244/14: Appl. 06/25/2010: publ. March 20, 2012 / V. G. Skopichev, A. V. Shchegolkova, M. A. Kucherenko; applicant State educational institution of higher professional education "St. Petersburg State Medical University named after academician I.P. Pavlov" of the Federal Agency for Health and Social Development, Institution of the Russian Academy of Medical Sciences Research Institute of Obstetrics and Gynecology named after. BEFORE. Ott of the North-Western Branch of the Russian Academy of Medical Sciences. – EDN VWXWPT.
Review
For citations:
Panova N.A., Vasilyeva S.V. Analysis of the efficiency of a rapid test for the early diagnosis of mastitis in cows. Legal regulation in veterinary medicine. 2022;(4):127-130. (In Russ.) https://doi.org/10.52419/issn2782-6252.2022.4.127
Views:
168
Email this article 