Evaluation of the real-time PCR method for the indication of Ureaplasma diversum in clinical and pathological material from cattle

Cultural and molecular biology methods may be useful for detection Ureaplasma diversum in the clinic matherial. Thus the methods of molecular biology are more sensitive and specific in comparison with cultural methods of ureaplasma identification. The purpose of this study was to compare the two methods for U. diversum identification, including the detection of U. diversum DNA by the diagnostic system “Ureaplasma diversum Amp”, based on real-time PCR and isolation of U. diversum on “UREAPLASMA-sreda” medium. “UREAPLASMA-sreda” was adopted for U. urealyticum amd designed to detect ureaplasma in clinical material from human. Comparing the results of real-time PCR with the results of grow on “UREAPLASMA-sreda” medium, it was found that real-time PCR is more sensitive than the culture method. In the case of a study of a clinical material, such as smears from the mucous membranes of the nasal cavity and the reproductive tract, real-time PCR reveals U. diversum, while inoculation “UREAPLASMA-sreda” medium by clinic sample doesn’t allow to achieve interpretable result due to a side of microflora growth. It was found that real-time PCR with “Ureaplasma diversum Amp” test system is more sensitive than inoculation in “UREAPLASMA-sreda” medium for the pathological material, and, unlike the nutrient medium “UREAPLASMA-sreda”, it is useful for a study of clinical material of various types from cattle.

Ureaplasma diversum, заболевания репродуктивной системы КРС, ПЦР, ПЦР в реальном времени, Ureaplasma diversum, diseases of the reproductive system in cattle, PCR, real-time PCR

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