One of the priorities of the state policy at present is to ensure food security. Its achievement is impossible without qualified personnel, whose effective work should be facilitated by a developed regulatory framework in the field of labor protection. An important role in ensuring food security is played by veterinarians, who, by carrying out prevention and treatment of animals, directly affect the health of citizens. However, the current situation with the regulatory support of their labor protection does not look encouraging.
Consideration of the documents in force in this area shows significant omissions: all veterinary specialists performing various functions are united in a single professional standard, there is no special approach to conducting medical examinations of veterinarians, there are no specialized labor protection rules for veterinarians – in the relevant rules for the agricultural sector, veterinars are mentioned only indirectly, their activities are not the subject of consideration.
Based on the study of the regulatory framework, it is concluded that the current level of regulation of labor protection of veterinarians is insufficient.

Federal Law No. 209-FZ of July 24, 2009 “On hunting and on the conservation of hunting resources and on amendments to certain legislative acts of the Russian Federation” establishes the basis for regulating the number of hunting resources, which are specified by subordinate regulatory legal acts and norms of veterinary legislation.
The article discusses the requirements that determine the procedure for organizing and carrying out activities to regulate the number of hunting resources, analyzes the relevant legal documents and statistical data, and provides a table developed by the authors of the results of hunting resources regulation in all districts of the Leningrad Region based on the results of 2022.

The article highlights some problems and shortcomings in Russian legislation in the legal regulation of biological waste management, and attempts are made to develop recommendations for improving veterinary legislation in the issue under study.
The lack of a clear understanding of what wastes are biological wastes, corresponding to a clear, complete, legally fixed definition, causes incomplete implementation of control and supervision measures and improper handling of biological wastes, which in turn not only increases the possibility of not bringing unscrupulous people to liability in matters of biological waste management of organizations and individuals, but also increases the risks of the emergence and spread of animal and human diseases. After analyzing the current regulatory legal acts regulating the management of biological waste in the Russian Federation, we can say with confidence that at the legislative level it is necessary to expand and specify the concept of "biological waste". Due to the lack of a complete, understandable, normatively fixed list of biological waste, difficulties arise in the preparation of accompanying veterinary documents, including in electronic form. Many specialists in the veterinary service experience "confusion" when using the terms "utilization" and "destruction" of biological waste.

In the conditions of modern development of animal husbandry, one of the important tasks is to obtain healthy viable offspring, the implementation of which is hindered by various diseases, both mothers and the young born by them. One of the little-studied diseases affecting both the health of the mother and offspring is genital mycoplasmosis. The aim of the study was to study the use of the antibiotic tulatromycin in pregnant cows with genital mycoplasmosis and its effect on the morbidity and weight gain of calves born by them. Experimental data show that the use of tulatromycin in cows with genital mycoplasmosis 40 days before delivery has a significant positive effect on the vital characteristics of their offspring. So, in comparison with calves born to cows that were not treated, the offspring from a group of cows receiving tulatromycin have a sharply reduced infection rate, there is a partial recovery of body weight and their morbidity decreases in the first month of life to the level of calves from healthy cows.

This review article is devoted to the problem of the emergence and spread of antibiotic-resistant enterobacteria producing beta-lactamase in horse breeding.
Foal colibacillosis is an acute infectious disease of young animals, manifested by diarrhea, signs of severe intoxication and dehydration of the body, caused by pathogenic Escherichia coli. Foals get sick in the first 3-7 days after birth. Infection occurs alimentary, less often – aerogenic and intrauterine transmission. The source of infection are sick and recovered animals. The duration of the disease is 2-3 days with a mortality rate of up to 90%, which leads to multiple economic losses.
Multiresistant Escherichia coli can accumulate in the intestines of healthy animals and be excreted with fecal matter into the environment, which leads to the spread of pathogenic microflora among livestock. In this case, the animals are latent or hidden carriers that do not show clinical signs of the disease.
Clinically significant isolates are causative agents of infectious and inflammatory diseases. These microorganisms represent a potential hazard for the development of an infectious process, they exhibit virulent properties that lead to damage to the tissues of the host organism, which significantly limits the possibility of their eradication.
Along with clinically significant isolates, there are colonizing isolates that do not cause infection at a specific site. It should be noted that under certain conditions, the colonizing isolate can cause an infectious process.
Irrational use of antimicrobials is a key reason for the development of tolerance. Also, the untimely detection of enzyme inactivation of antibiotics by laboratory methods and the spread of resistance genes play an important role. Currently, disks and media with antibacterial drugs are widely used to detect multidrug resistance of microorganisms. Along with these methods, PCR diagnostics and sequencing are used to accurately determine antibiotic resistance genes.
Due to the importance and relevance of this topic, many scientists are conducting research to detect and study the multiresistance of enterobacteria such as E. coli, and their spread among animals.

The article is devoted to the differential diagnosis of strains of Clostridium perfringens and Fusobacterium necrophorum in the studied material of cattle. The research topic is relevant, since rational, timely and accurate diagnosis of the etiology of infectious diseases in cattle in the conditions of industrial cattle breeding prevents the occurrence and transmission of infectious agents, thereby ensuring cost-effective uninterrupted production of highly nutritious products and raw materials.
Timely and accurate diagnosis allows veterinarians to prescribe an effective treatment and prevention plan to prevent the occurrence and spread of infectious diseases in cattle, including intestinal diseases [2,6]. One of the causes of hemorrhagic enteritis, reduced productivity and mortality from 25% of young animals may be enterotoxemia associated with toxin-producing strains of Clostridium perfringens. Differential diagnosis is an obligatory step in a comprehensive diagnosis, taking into account epizootological data, clinical signs of sick and conditionally sick animals, pathoanatomical data of forcedly killed and dead animals, laboratory diagnostic methods, including bacteriological and molecular genetic methods.
As a result of the study, optimal methods of laboratory diagnostics were applied, including bacterioscopy, the method of pure cultures and polymerase chain reaction (PCR), which made it possible to identify strains of Fusobacterium necrophorum and Clostridium perfringens. Accurate and rapid identification of pathogens Fusobacterium necrophorum and Clostridium perfringens will allow veterinarians to develop a plan of treatment and preventive measures in a short time.

For successful pharmacotherapy of animals, it is important to identify species of Enterococcus faecalis pathogens isolated from milk in infectious mastitis of cows, analyze the antibiogram and interpret the results, and laboratory control over the mechanisms of antibiotic resistance. This is necessary for veterinarians to rationally select antibacterial drugs and predict their clinical effectiveness.
The purpose of the study was to study the resistance to antimicrobial drugs of Enterococcus faecalis bacteria isolated from milk in infectious mastitis of cows.
In the period from 2021 to 2022, 100 strains of microorganisms were isolated from the mastitis milk of cows. The obtained strains were identified as gram-negative bacteria in 32% of isolation cases, gram-positive bacteria - 68%, of which 14% were Enterococcus faecalis.
When interpreting the antibiogram, most isolates of Enterococcus faecalis are sensitive to tetracycline in 21.4% of isolation cases, ampicillin, amoxicillin in 100%. It was noted resistant to tetracycline in 78.6% of cases of isolation, gentamicin, cephalexin, cefotaxime, erythromycin, clindamycin, lincomycin, tobramycin, ceftriaxone and streptomycin in 100%.
Antibiotic resistance correlates with the clinical failure of antimicrobials in veterinary medicine.
Multi-resistance of Enterococcus faecalis to various groups of antibacterial agents leads to limited use of antibiotics, which significantly reduces the possibilities of therapy and affects the economic condition of agriculture.

Microbial spoilage of food products leads to significant losses with negative social and economic consequences. In order to better control contamination and microbiological spoilage of food products, a new GOST P 70354-22 has been introduced. Meat and meat products. General requirements and procedure for testing to justify expiration dates (publication date 09/29/2022). Great importance is attached to the identification and quantification of the additional microbiological indicator Brochothrix termosphacta in meat, meat and meat-containing products packed using vacuum or modified gas atmosphere. Brochothrix sp. may become the dominant type of meat spoilage in MHA packaging and vacuum packed in the presence of sufficient oxygen. Brochothrix causes mucus and stickiness of the meat surface, unpleasant odors. Brochothrix also causes mucus and stickiness on the meat surface. GOST P 70354-22 describes a method for detecting and counting the number of bacteria Brochothrix sp. For their cultivation, a dense selective medium STAA was proposed. It allows you to isolate, identify and determine the amount of Brochothrix in samples. The technique described in GOST P 70354-2022 was tested. According to the results of cultivating primary crops, we observed the growth of characteristic small, round, shiny, gray-white colonies of Brochotrix on the surface of a special medium. The studied bacteria were oxidase-negative and catalase-positive. 

In view of the increasing interest in the issues of antibiotic resistance of opportunistic microorganisms, there is a need for a closer study of bacteria isolated from animal products. Such bacteria can carry genes for resistance to antimicrobial drugs, including those used to treat human diseases [4, 10]. In this case, drinking milk is of particular interest, because the antibiotic-resistant bacteria contained in it can freely enter the body in the absence of additional heat treatment before consumption.
The work took into account the requirements of the Technical Regulations of the Customs Union (TR CU) 021/2011 "On food safety" and TR CU 033/2013 "On the safety of milk and dairy products", as well as the recommendations of the European Committee for Antimicrobial Susceptibility Testing EUCAST and the Interregional Association for clinical microbiology and antimicrobial chemotherapy MACMAC.
As a result of the research, the presence of resistance to antibacterial drugs was determined in 36 bacterial isolates from milk sold in St. Petersburg and the Leningrad Region. It was found that the most common resistance to ampicillin (24 cases) and tetracycline (6 resistant isolates, 8 with intermediate values). There were also cases of resistance and intermediate results of isolates in relation to other antibiotics, including cephalosporins.

We conducted bacteriological study of samples of small-sized beef, as well as minced pork, beef, homemade, purchased in retail chains. Bacteriological, bacterioscopic and proteomic research methods were used. The total bacterial contamination, freshness indicators, the presence of pathogens of bacterial diseases, opportunistic microorganisms and representatives of the dominant saprophytic microflora were determined. The absence of Salmonella, listeria, Pseudomonas aeruginosa, Staphylococcus aureus, Proteus, yeast and molds was established. The dominant microflora of meat are Enterobacteria of the genus Citrobacter, Entrrococcus faecium.

Increasing gross milk production is the main task of the dairy cattle industry. The level of milk productivity of a cow depends on the age and living mass of the calf at the first insemination. Under certain conditions, the technology of herd reproduction in the conditions of cattle breeding in a particular farm (maintenance technology, feeding conditions, etc.) it is necessary to determine the optimal period of the first fruitful insemination of heifers.
The productivity of Holstein cows was studied due to the age of the first insemination. Among the first calves inseminated at the age of 11-18 months, cows turned out to be more productive with an age of insemination of 14 months. In order to increase productive longevity, it is recommended not to inseminate the calves earlier than this age.

The aim of the study was to establish the degree of influence of the Biolatic G–500 probiotic on the biochemical parameters of the blood of calves with nonspecific bronchopneumonia. The object of the study is calves of a black–and-white Holstein breed aged 20-30 days. Groups of animals were formed as they were born and became ill. The first (control) – intact – group (n=10) was formed from clinically healthy animals. The second (n=10) and third (n=10) experimental groups included animals with nonspecific catarrhal bronchopneumonia. The animals of the second group received a typical treatment regimen, including: a single subcutaneous injection of the antibiotic "Pulmovet" at a dose of 1.0 ml per 40.0 kg of body weight; intramuscular injection of the antibiotic "Florox" at a dose of 1.0 ml per 15.0 kg of body weight with an interval of 48 hours before recovery; with an increase in body temperature, "Ketoprofen 10%" at a dose of 3.0 mg per 1.0 kg of body weight, once a day before its normalization; with severe shortness of breath, a single intramuscular injection of Dexamethasone at a dose of 2.0 ml per head. Animals of the third group were additionally prescribed the probiotic drug "Biolatic" (Biolatic) G-500 at a dose of 10.0 g per day per head. It was found that the use of Biolatic G-500 probiotic in the treatment regimen of nonspecific catarrhal bronchopneumonia of young animals contributes to the acceleration of normalization of the biochemical status of animals. This is evidenced by the dynamics of the digital values of its main indicators, in comparison with intact animals and animals whose treatment regimen did not include this drug.

Surgical resolution of comminuted fractures of tubular bones is an urgent issue for practicing veterinarians. Comminuted fractures account for 52%, transverse fractures for 8.7%, oblique fractures for 26.1%, and compression fractures for 13.0% of total canine fractures. Thus, comminuted fractures of the tubular bones of the extremities are the most frequent.
There are several types of bone fixation in comminuted fractures. Many authors do not recommend using of intramedullary osteosynthesis in the treatment of this type of fracture since it is not possible to connect all bone fractures. The aim of this study was to compare the methods of surgical treatment of comminuted fractures in dogs and cats admitted to the clinics and to choose the most optimal ways of treatment. In total, 4,396 cats and dogs were admitted to the clinic, excluding those admitted for vaccination. The surgical department received 35% of the total number of animals. Patients with bone and joint pathology accounted for 19% of all surgical causes and to 7% of all animals admitted to the clinic. In the group of 9 cats and 9 dogs three main types of fracture were diagnosed: transverse fracture in 6 animals (4 dogs and 2 cats), oblique fracture in 4 animals (3 dogs and 1 cat) and comminuted fracture in 8 animals (2 dogs and 6 cats). The article is illustrated with X-rays images of the clinical cases presented. In order to perform osteosynthesis using a single-plane bilateral external fixator, the authors injected 2-3 Kirchner spokes each into the proximal and distal bone fragments by drilling through the bone while perforating soft tissue on both sides. The ends of the spokes on both sides were bent towards the opposite fragment, parallel to the bone axis, at a distance of 1.0 to 3.0 cm from the skin surface. The ends of the spokes form the rods of a bilateral single-plane fixator. The rod-forming spokes are tightened with wire serrations on 3-4 levels and additionally fixed with fast hardening plastic (turbocast) or bone cement (Palacos, GMW, Osteobond) while controlling the fracture reposition. Combination with wire serclages, sutures, compression screw or intramedullary Kirschner wire osteosynthesis is possible.

The aim of this study was to investigate the effect of popular non-inhaled and inhaled general anesthetics on the respiratory function of dogs during operative delivery. Experimental studies have been performed on pregnant bitches that have been subjected to caesarean section. The selection of animals was carried out according to pre-selected criteria, the age of the animals participating in the experiment was from 2 to 6 years, the average body weight was 22 ± 4.6 kg. Animals were divided into 3 groups. Dogs in the first group received intravenous anesthetic propofol at a dose of 4-6 mg/kg for induction and maintenance of general anesthesia. he dogs of the second group used an inhaled halogen-containing anesthetic isoflurane 0.5–2.0 vol% for the administration and maintenance of anesthesia. For the induction and maintenance of general anesthesia in animals of the third group, a more modern inhalation anesthetic sevoflurane 1.0–2.0 vol% was used. Each group included 20 animals. Analyzing the results obtained on the study of the functions of external respiration of dogs during caesarean section under general anesthesia, it can be argued that the most significant changes in the studied parameters occurred at the initial stages of anesthesia with propofol, which are associated with an increase in the concentration of this anesthetic in the blood and a more pronounced depression of the respiratory center compared to with inhalation anesthetics.

Absolutely all preclinical studies involve three groups of experiments: preclinical studies of the pharmacokinetics of the substance, including toxicokinetics, preclinical studies of the pharmacokinetics of the drug and the clinical study of pharmacokinetics on target animals. Pharmacokinetic studies of native drugs are complex because they usually involve the administration of complex components. Thus, only the use of the whole complex of pharmacognostic, chemical, spectral, physicochemical and biological methods can provide an objective assessment of the authenticity and quality of medicinal herbal raw materials and medicinal herbal preparations. The study used Wistar white rats, weight 150-160g according to the Guidelines for Evaluation of Medicinal Products (3).
The purpose of the study was to evaluate the pharmacokinetic properties of a new feed additive in the form of a premix of sorbents and phytocompositions: milk thistle (DV-silibinin, Pharmcenter VILAR CJSC), thyme (DV-timol, Akhma100). Supplements in the form of finished dosage forms were standardized for the active substance, and have a constant composition. Thus, it allowed to create a study design for a new substance in one animal species. Since the composition of the sorption component was studied earlier, the introduced substances were considered separately and in combination with sorbents.
After a single injection, the linearity of the pharmacokinetics was assessed, based on the administered different doses, the analysis of the main biochemical parameters of the blood was carried out, and the bioavailability was also calculated. Thus, the new premix based on sorbents and plant substances in the form of milk thistle and thyme does not have a negative effect on the body of laboratory animals. As a result of the studies, LD50 and LD100 were not established for the drug, the drug is classified as low-toxic, belongs to the fourth hazard class according to GOST 12.1.007-76 "Harmful substances", which allows further toxico-therapeutic tests.

Bentonite clays, especially those with a high content of montmorillonite, are widely used in the oil refining, winemaking, agricultural, and cosmetic industries. Such a wide and multidirectional use is primarily due to their unique physicochemical properties. The presence of these properties in bentonites arouses interest in them in pharmacy as excipients (emulsifiers, stabilizers, structure formers, etc.) as part of various dosage forms. However, despite the direct mention in the current State Pharmacopoeia of the Russian Federation of the possibility of using bentonites as excipients, a substance officially permitted for these purposes in Russia is not registered and is not produced. In this regard, the urgent task was to conduct a preliminary pharmaceutical assessment of bentonite from a domestic field for the potential possibility of using it in veterinary pharmacy. The study was carried out on a modified sodium form of bentonite, processed to a homogeneous fine powder in comparison with the original alkaline earth form. The assessment was carried out according to indicators for pharmacopoeia analysis: description, authenticity, pH of an aqueous suspension, weight loss on drying, the content of arsenic, iron, calcium, carbonate chlorides, the presence of coarse particles, microbiological purity, swelling, gelling, emulsifying ability and stability emulsions. The evaluation of the emulsifying ability, emulsion stabilization, gelling properties and a number of pharmacopoeia indicators of good quality demonstrated the potential possibility of using domestically deposited bentonite in veterinary pharmacy (using the example of the Kantemirovskoye deposit in the Voronezh region) as excipients in the composition of dosage forms for veterinary use, in particular, heterogeneous ointments. In this regard, the sodium modification of bentonite seems to be more promising for further in-depth study.

Lymphoma in cats is a malignant neoplasm of lymphoid tissue (lymphocytes and other cells of the defense system are affected). Causes of lymphoma in cats can vary, including genetic factors, viruses, and exposure to carcinogens. Most often, patients are admitted to an appointment already with an advanced stage of lymphoma, since the signs of oncological disease are nonspecific and can be easily confused with other diseases of the gastrointestinal tract. Symptoms in cats may include swollen abdominal lymph nodes, decreased appetite and loss of lean body mass, behavioral changes (lethargy, weakness), vomiting, and fever. Lymphoma in cats accounts for 50-90% of all formations of the hematopoietic system. Chemotherapy is one of the main treatments. It consists in the use of cytostatics that inhibit the growth and division of tumor cells. Increasingly, new protocols are being tested in the treatment of animals for various oncological diseases. The length of the chemotherapy protocol varies according to the stage of the disease, clinical manifestation and extent of the lesion, but the most effective chemotherapy protocol is CHOP (endoxan, doxorubicin, vincristine, prednisolone), which is designed for 25 weeks in cats. There are data on the use of the drug etoposide not only in humans but also in animals, which can be effective and serve as a replacement for endoxan in the CHOP treatment protocol. But in addition to the main methods of treatment, it is important to monitor the general condition of the animal, since the main goal of chemotherapy is to prolong the normal quality of life of the animal.

The article presents the results of studies of subchronic oral toxicity in laboratory animals of the new veterinary drug Amoxiantar. The studies were carried out as part of a laboratory-experimental (preclinical) study of a medicinal product with the aim of introducing it into clinical veterinary practice. Our goal was to study the effect of Amoxiantar on the hematological and biochemical parameters of the blood of laboratory animals. Three experimental groups were formed, of which two experimental and one control, consisting of white outbred rats, 10 animals each. The drug was administered to animals daily intragastrically for 15 days, rats of the first experimental group at a dose of 150 mg/kg body weight, rats of the second group 15 mg/kg body weight, the control group was injected with distilled water. Blood was taken on the first and tenth days after the completion of the administration of the Amoxiantar preparation, on the 16th and 25th day of the experiment, respectively. Statistical processing of the results of the general clinical analysis of the blood of animals on the tenth day after the completion of the drug (25 days of the experiment) revealed monocytosis in the blood of the first and second experimental groups and leukocytosis in the first experimental group compared with the control group, however, these values were normal rat blood parameters. The revealed statistically significant differences, on the 25th day of the experiment, between the second experimental group and the control group in terms of AST, alkaline phosphatase and creatinine were clinically insignificant. During the 25th day of the subchronic experiment, the new veterinary drug Amoxiantar did not adversely affect the hematological and biochemical blood parameters of laboratory animals at doses of 15 and 150 mg/kg of animal body weight.

The purpose of the research was to study the local irritating effect of the drug Tildox on the skin. Tildox is a complex antibiotic in the form of a powder. Tildox contains the active ingredients doxycycline hyclate - 100 mg and tylosin tartrate - 100 mg in 1 g of the drug and excipients.
In the study 20 New Zealand White rabbits weighing 3.2 - 4.1 kg were used. Prior to testing, rabbits were shorn in 10 x 15 cm patches on both sides of the back. Animals were divided into 2 groups of 10 animals each. Rabbits of the first group were given applications using a 10% aqueous solution of the antibiotic, rabbits of the 2nd group were treated with a 50% aqueous solution of the drug. Applications were carried out 1 time per day for 14 days at a dose of 0.5 ml per animal. Aqueous solutions of antibiotics were applied to the right side of the rabbits, the left side of the rabbits served as a control: applications with water were carried out in this place. The exposure time each day was 4 hours.
As a result of the research, it was found that after repeated application of the drug Tildox in 10% and 50% concentrations by applications 1 time per day for 14 days at a dose of 0.5 ml, no changes in the skin were observed per animal.

The main task of preserving populations of small breeds of horses imported from abroad with their unique characteristic features of the exterior and type of constitution is the organization of the reproduction of the livestock and the preservation of the exterior parameters of the breed in descendants. In modern conditions of a small number of populations of such breeds, the influence of the inbreeding depression factor increases, as well as the manifestation of recessive mutations and hereditary pathologies in the offspring.[8] On the one hand, this can form changes that are not typical for the breed: phenotype, measurements, body type of the animal's format; on the other hand, such features of breeding horses in the absence of a selection press and selection will lead to the formation of a population of horses of the breed type. Such breed types should be fixed by breed leaders and have a territorial localization; if this does not happen, then the livestock that does not meet the requirements of the breed may lose the status of purebred. [2][6]
The study consisted in monitoring the indicators of measurements by generation to identify the dynamics of changes in the format of the horse population and their compliance with the breed standard of American miniature pony horses. The article presents the indicators of the main measurements of American miniature pony horses for a number of generations and carried out their comparative analysis, with the standard requirements of the corresponding measurements of the breed. The analysis showed the following dynamics: in height at the withers, the increase is 46.5%, in the oblique length of the body, the increase is 97.8%, in the distance from the withers to the tail, the increase was 103.5%, which characterizes a more intensive growth of the peripheral skeleton in the postembryonic period and the formation of an elongated format with age . While the angle of the shoulder, wrist and tarsus is less variable in the age aspect and depends on individual hereditary factors, and not on age. A number of recommendations have been developed for specialists of the club "IDALGO" on the selection of parental pairs for correction for compliance with the standard requirements for measuring indicators in offspring.

The article presents data on the study of modern methods of keeping song and ornamental birds at home. Creation of optimal microclimate parameters for birds based on their families and species. Creating increased attention to acquainting people with the world of song and ornamental birds and ways to attract them through the thoughtful placement of artificial nests in apartments, courtyard parks and gardens, competent feeding of birds in different seasons of the year in the general context with environmental issues in large metropolitan areas. Keeping and breeding song and ornamental birds at home makes it possible to preserve many species, and with proper care and feeding, birds live in captivity for a long time and breed well.

The article presents the results of monitoring mycotoxins in compound feeds for productive animals. Mycotoxins are secondary metabolites of microscopic fungi with pronounced toxic properties. Since mold fungi spores are ubiquitous, and intensive crop cultivation technologies and environmental pollution disrupt the ecological balance, the problem of mycotoxicosis is global.
The purpose of the scientific work was to monitor and analyze the content of mycotoxins in compound feeds for productive animals sold on the territory of St. Petersburg and the Leningrad region.
The research was carried out on the basis of the Department of biological safety of feed and veterinary drugs of the North-Western Testing Laboratory of the FGBI "VNIIZH". 40 samples of complete compound feeds for productive animals sold on the territory of St. Petersburg and the Leningrad region were used as research material.
The studies were carried out in stages. At the first stage, the total toxicity of feed was determined according to GOST 31674-2012 "Feed, compound feed, feed raw materials. At the next stage, a mycological study was carried out of those samples that, according to the results of biotesting, had toxic properties. Then, to determine the exact cause of the toxicity of the samples, enzyme immunoassay was performed using indirect solid-phase competitive ELISA.
Thus, analyzing the data obtained, it can be concluded that four samples of compound feeds out of 40 were contaminated with mycotoxins: deoxynivalenol and zearalenone simultaneously. This is due to the fact that the producer for these mycotoxins is one type of microscopic fungi - Fusarium graminearum, which turned out to be affected by these samples of compound feeds. It should be noted that these mycotoxins are characterized by low toxicity and a high degree of accumulation in the body, which contributes to the occurrence of chronic poisoning, therefore, the need for further monitoring of the welfare of the feed base for farm animals and poultry in veterinary and sanitary terms increases.

Okkervil is one of the small rivers of St. Petersburg, the source of which is located on the territory of the Leningrad Region, and the mouth is within the city of St. Petersburg. The length of the river is 18 kilometers, and in its course it passes through several rapidly developing settlements, including the village of Novosergievka and Kudrovo. The Okkervil River and its coastal zone, both within the city and beyond its borders, are actively used for various purposes, including recreation and amateur fishing. However, like many other rivers flowing through the territories of large cities, the river. Okkervil is subject to diverse anthropogenic pressure, leading to water pollution. Thus, the water quality of this river may not be of adequate quality for use in fisheries and recreational purposes. The main objective of this work is to determine the chemical composition of the waters of the river and to establish its suitability for fisheries and recreational water use.
Sampling was carried out during the open water period at three points with different types of anthropogenic load: outside the city, near the village of Novosergievka; at the entrance to the city; and in the residential area, near the Prospekt Bolshevikov metro station. At each point, the main hydrochemical indicators were determined: the concentrations of iron, copper, ammonium ions, phosphates, nitrates and nitrites, heavy metals, as well as the concentration of dissolved oxygen and BOD5.
At all sampling points during the research period in the river, the standard values for the content of nitrites, phosphates, iron and copper were exceeded.The results obtained indicate a high level of pollution associated, among other things, with the ingress of untreated storm drains into the river, and untreated and undertreated household waste. The highest level of water pollution was noted at a point located in a residential area. The water here corresponded to the V class of quality (dirty). Both in the spring-summer and autumn periods of the study, the water of the Okkervil River in the other two sections corresponded to III-IV quality class (moderately polluted - polluted). The water quality of the Okkervil River deteriorates from the source to the mouth. In all the studied areas, the river is unsuitable for fisheries and recreational water use.

The objectives of this review were to analyze the ways of spreading rodents, what kind of danger and harm they pose, as well as what methods are used to combat them. The article provides brief information about the distribution of mouselike rodents and the influence of factors on the distribution and dynamics of populations. The issue of transmission and storage by rodents of an infection that poses a danger to humans and their pets is considered. The methods of combating mouse-like rodents are also analyzed and the most used active substances that are part of rodenticidal agents are identified. The number of rodents can vary significantly, so the seasonal dynamics in a period unfavorable for the life of the population is at low levels, and with the improvement of habitat conditions and food supply, the number of animals increases significantly. Analyzing the factors influencing the rodent population, it is possible to predict the number of animals and determine the optimal timing of deratization work. Currently, there are a significant number of rodenticidal drugs, the effectiveness of which has been confirmed in practice.

The aim of the conducted studies was a comprehensive assessment of the clinical and physiological state of laying hens, which included a fish feed additive (DCR) in the main diet. The composition of the new Prinarovskaya DKR includes chilled river fish, its processed products and grain products (wheat bran). The additive preparation process goes through several stages: grinding, cavitation heating and subsequent drying of the resulting mixture. The investigated additive is a homogeneous dry mass and contains a number of amino acids, vitamins and minerals in its composition. DKR "Prinarovskaya" was fed to laying hens of the Lohmann Lsn-Classic breed of 130 days of age. All chickens participating in the experiment were divided into 4 groups. Chickens of the 1st experimental group included 8 g of "Prinarovskaya" additives per 100 g of the main diet; chickens of the 2nd experimental group - 6 g of additives to OR; chickens of the 3rd experimental group – 4 g of additives to OR; 4-control group of chickens were fed only OR – compound feed PK1-1G-1227, prepared in accordance with GOST R 51851-2001. The usefulness of poultry feeding is reflected in the morphological and biochemical parameters of blood, which served as the basis for using them in an experiment to characterize metabolic processes and assess the state of the body of laying hens. An important indicator of the effect of the new feed additive "Prinarovskaya" on metabolic processes was the determination of the concentration of total protein and the main protein fractions (albumins, globulins). The state of lipid metabolism in terms of cholesterol and triglycerides was analyzed. Indicators of carbohydrate metabolism and the functional state of the liver were also studied. To characterize the mineral metabolism, the level of calcium and phosphorus in the blood serum was determined. The conducted study with a comprehensive assessment of morphological and biochemical parameters of blood confirmed the expediency of including Prinarovskaya DCR in the diet of laying hens, which generally has a positive effect on the state of their body.

Today, the topic of assessing the toxicity of feed is relevant. In Russia, the production of environmentally friendly products is one of the priority areas in agriculture. Eating feed contaminated with industrial waste, pesticides, mycotoxins by domestic and farm animals can adversely affect their health. In this work, the quality of mixed grass hay was assessed by biotesting using Paramecium caudatum test organisms. The essence of the method lies in the preparation of aqueous extracts and aqueous solutions of acetone extracts of the sample and the effect of the obtained extracts on Paramecium caudatum. This method is convenient because you can quickly get the necessary results regarding the toxicity of the sample. The study was carried out on the basis of the laboratory of the Department of Biochemistry and Physiology of the St. Petersburg State University of Veterinary Medicine. Less than 50% of Paramecium caudatum survived in an aqueous extract and an aqueous solution of an acetone extract, the toxicity of the sample was established according to GOST 31674- 2012 «Feeds, compound feeds, material for compound feeds. Methods for the determination of common toxicity»

Estimates of the breeding value of producers and breeding stock of cattle use various information and analytical models and statistical methods, including the determination of the breeding value of animals using the BLUP methodology and the BLUP AM methodology, for the subsequent transition to predictive genomic SNP selection. The study was conducted on the reproductive population of cows of the Kholmogory breed of the Arkhangelsk region, Kholmogory district. A genealogical analysis of a spontaneous population of cows of first-calf heifers of the Kholmogory breed was carried out, represented by the offspring of 66 bulls, which were evaluated according to the main productive indicators and breeding value (PC). A rank assessment of sires was carried out according to the efficiency of the productive contribution of daughters, bulls' PC for milk yield and MJ, MDB. The studies confirmed the literature data on the possibility and effectiveness of using phantom cattle populations in assessing the breeding value of producers.

Studies have shown that the entry of metals and other pollutants by aerogenic means into Lake Ladoga is associated with the presence of both remote and local sources of entry into the atmosphere. In this regard, Lake Ladoga is in a vulnerable state, as studies have shown – since often the metal content in precipitation is higher than in water. This is especially dangerous for the shallow littoral zone of the lake, where the dilution rate is low due to the volume of water. This showed their presence in water, sediments and fish, and most importantly – the impact on the biota and, above all, on the ichthyofauna, since fish are able to accumulate pathological information during their life cycle. Toxicosis has become a mass phenomenon in these waters, which has affected the natural reproduction of fish with a prolonged effect on populations and ichthyocinosis in general.
The inflow of metals and other pollutants of organic nature (xenobiotics) in the form of dry and wet precipitation forms a polluting surface runoff, which is associated with difficulties in carrying out preventive measures. 

The industry in the Lipetsk region is quite well developed, including ferrous metallurgy, agriculture, construction, and transport infrastructure. Harmful emissions accompanying the work of industry and transport change the state of water, air, land, thereby having a negative impact on the health of humans and animals.
In order to eliminate negative consequences and improve control methods, the Lipetsk region, in the national project "Ecology", determined its participation in 6 federal / regional projects: "Clean Air", "Clean Water", "Preservation of Unique Water Bodies", "Preservation of Forest -sov", "Clean Country", "Integrated system of municipal solid waste management".
The aim of the work was to identify the main sources of environmental pollution in the Lipetsk region and to assess the existing methods for correcting the negative impacts from industrial enterprises. We have conducted studies of domestic literature sources, reports and reports in various databases and open sources, in the period from 2018-2022. During the study, the data of the Office of Industrial Ecology and the Hydrometeorological Center of the Lipetsk Region were analyzed for the release of harmful (hazardous) substances into the environment by heavy industry enterprises for 2020-2022.
To reduce emissions of pollutants in the Lipetsk region, the Federal project "Clean Air" is being implemented. According to the Federal Project, until 2024, the implementation of the planned activities and achievement of the targets during the implementation of the National Project "Ecology", including the reduction of emissions of toxins into the environment, the formation of a unified scheme for waste disposal, and the maintenance of cleanliness, remain the main priority task. and rehabilitation of water bodies, preservation of the population of a number of rare, especially valuable, endangered species of animals.

Pig breeding in the Russian Federation is one of the leading branches of agricultural products. In large industrial pig breeding complexes, part of the diseases in livestock is associated with the respiratory system. This is noted in statistical reports on newborn animals in pig breeding complexes of the North-Western region of the Russian Federation. The nasal cavity in animals performs a number of vital functions, which include heating and humidifying the incoming air. The work was performed at the Department of Animal Anatomy of the St. Petersburg State University of Veterinary Medicine. The corpses of Yorkshire piglets aged 10-14 days were used as the test material. In total, seven corpses of piglets delivered from the pig breeding complex of the Leningrad region were examined. Arterial vascularization of the nasal mucosa in Yorkshire piglets is carried out due to the cuneiform artery, which is a branch of the internal maxillary artery. In the studied piglets, the diameter of the cuneiform artery averaged 1.25±0.15 mm– In the nasal cavity, this artery passes through the cuneiform opening, and in the area of the last molar is divided into three branches. The first branch is the artery of the nasal septum, its diameter on average is 0.58 ± 0.05 mm; the second branch is the artery of the ventral nasal conch, its diameter on average is 0.73± 0.06 mm; the third branch is the artery of the dorsal nasal conch, its diameter on average was 0.54± 0.04 mm. Arterial branches of the first, second and third order in the mucous membrane of the nasal septum throughout have a constant diameter and are located parallel to each other.

The article presents the results of studying the effect of long-lived radionuclides 137Cs and 90Sr on some biochemical parameters of the blood serum of pigs. Ionizing radiation has a pronounced biological effect, and the radiobiological reaction in pigs begins with the formation of various kinds of cellular damage as a result of the transfer of radiation energy to them. A feature of the influence of incorporated irradiation on the body of pigs is not only decay, but also the formation of highly reactive ions and free radicals, which are involved in the primary radiation-chemical processes in organs and tissues with the formation of toxic metabolites. The severity of the damage depends on the type of radiation exposure, the power and dose of radiation, radiosensitivity and the age of a particular animal species. In addition, the tropism of long-lived radionuclides and the level of radioactive doses received for various organs and tissues of pigs are of particular importance.

Such a breed of dog as the Russian Hound is the most popular breed among hounds in Russia. The average weight is 25 -35 kg, and at the withers it reaches up to 65 cm, representatives of this breed live up to 14-16 years. They are easily trained and are ideal hunting companions for hare and fox, during which they often suffer injuries in the chest cavity, and veterinarians face the difficult task of providing surgical care to such patients. Knowledge of the anatomical and topographic features of the arterial bed of the lungs is necessary when providing surgical care. During the formation of the functional activity of the body, the change in the diameter of the arterial bed is of particular interest. The purpose of the study was to study the arterial bed of the lungs of the Russian Hound breed in the age aspect, to give the arteries a morphometric characteristic. Three age groups of dogs were selected for the study: newborn puppies, puppies at the age of nine months, and mature dogs. Fine anatomical preparation, vasorentgenography, morphometry was chosen as research methods. During the study, morphometric data of such vessels as: pulmonary trunk, right and left pulmonary arteries, right and left bronchial arteries were obtained.

This article presents the results of a screening study of the blood serum of cows for the content of carotene in the period of 28-62 days after calving. Out of 31 samples, the results of six samples corresponded to the normative intervals, which amounted to 19.35%. In the remaining animals, carotene was determined in the range from 0.06 to 0.34 mg/dL. In accordance with the obtained data on the concentration of carotene, samples were taken from the sample and 3 groups of animals were formed in such a way that, in an intergroup comparison, Student's t-test was less than 0.001. In the blood serum, a study was made of the concentration of vitamin A, vitamin E and beta-hydroxybutyric acid. Studies have shown that the lowest values of carotene correspond to the minimum concentrations of vitamins A and E. An increase in the level of carotene by 2.36 times to 0.26 ± 0.01 mg / dl is accompanied by an unreliable increase in the concentration of vitamins A and E by 33.3 and 12.8 %, respectively. The highest concentration of carotene in animals of the third group (0.45±0.02 mg/dl) contributes to an increase in the content of fat-soluble vitamins in the blood - A (two times) and E (1.65 times), compared with the indices of cows of the group 1 (both P<0.001).
When considering the content of beta-hydroxybutyric acid in cows, it can be noted that with carotene values below the physiological range in groups 1 and 2, an increase in its concentration above the reference values is observed, while the highest level of carotene corresponds to the concentration of beta-hydroxybutyrate within the normative limits and below, than in groups 1 and 2 by 51.6% and 59.1% (P<0.05). The conducted studies made it possible to determine the high frequency of occurrence of a reduced level of carotene in cows during the milking period, which amounted to 80.65%. The concentration of carotene below physiological limits leads to a decrease in the concentration of important fat-soluble vitamins in the blood, and also contributes to the development of ketonemia.

The aim of the study was to assess the impact of prolonged irregular search and rescue operations on the hematological blood parameters of service dogs of medium breeds.
The study was conducted on dogs of medium breeds: Border Collie, Australian Shepherd, Nova Scotia Retriever. The age of the animals ranged from four to ten years, and the body weight from 16.2 to 22.0 kg. Assessment of the condition of the animals before and after their participation in search and rescue operations was carried out based on the results of a clinical examination and a clinical blood test. A general blood test was performed on a Mindray BC-2800 Vet hematological analyzer. The following indicators were determined: the number of leukocytes; erythrocytes; hemoglobin level; erythrocyte sedimentation rate (ESR). The leukogram was derived from stained blood smears using an OLYMPUS CX23 Evidence immersion microscope. Based on the data obtained during the physical examination, there were no signs of any diseases, all the animals were clinically healthy. At the same time, the occurrence of obvious stress was noted in all. Blood sampling from animals was carried out after at least eight hours of a starvation diet, from the lateral subcutaneous vein of the thoracic limb, taking into account compliance with the rules of asepsis and antiseptics.
It was found that the loads during prolonged irregular search and rescue operations have an impact on the clinical picture of the blood, expressed in a decrease in the average number of erythrocytes, hemoglobin, rod-shaped neutrophils, lymphocytes, and monocytes, as well as an increase in the number of leukocytes, eosinophils and neutrophils, as well as an increase in the rate of erythrocyte sedimentation.

In 2021, in the vivarium of the FNC "VNITIP" of the Russian Academy of Sciences, an experiment was conducted on 4 groups of broiler chickens of the SGC "Smena 8" breeding with the use of a mineral supplement in the form of Lasparaginates in the amount of 7.5% of the accepted norms, both with and without phytase. As a result, it was found that Lasparaginates and microbial phytase contributed to the enhancement of biological activity, providing better assimilation of metals. With a low proportion of lactobacilli in the intestinal flora of broiler chickens of all groups, there was a tendency to increase them in the fourth group of birds that received 7.5% of L-asparaginates from the accepted norms in combination with phytase in the diet.phytase in the diet. Among the cellulolytic bacteria in the intestinal microbiome, the highest rates were found in the first and third groups of broilers that received both an inorganic form of mineral supplement in the form of premixes (the first group) and organic, in the form of L-asparaginates in combination with phytase (the third group), which compensated for the low proportion of lactobacilli in the intestines of birds of these groups. The obtained results testified to the positive effect of the studied mineral supplement and phytase on the safety indicators, the live weight of broiler chickens and the microflora of their intestines.

The article presents the results of determining the amino acid composition of pork with quality defects, such as PSE, DFD, RSE, RFN and PFN. Meat is one of the traditional sources of high-grade protein, the nutritional and biological value of which depends on the ratio of essential and non-essential amino acids that make up these proteins. The nutritional value and amino acid composition of meat proteins are influenced by various factors, such as the type of animal and breed, gender and age, feeding, storage conditions and others.
As part of the implementation of the Food Security Doctrine of the Russian Federation, much attention is paid to improving the quality and rational use of meat and other slaughter products, including those with quality defects such as PSE and DFD. In addition to defects PSE (pale, soft, exudative) and DFD (dark, hard, dry) for pork, there are three more categories: PFN — pale, hard, non-exudative (pH 5.58); RSE — red, soft, exudative (pH 5.67); RFN — red, hard, nonexudative (pH 5.71).
The purpose of the research was to evaluate the amino acid composition of pork with quality defects.
The research was carried out in the conditions of the educational and research center for the examination of food and animal feed of the St. Petersburg State University of Veterinary Medicine in the period 2021-2022. The materials for the study were 66 samples of pork of various quality categories (11 samples of each category). For the tests, point samples were taken from different muscle groups, then a combined sample was made and sample preparation was carried out according to the M method-04-94-2021 "Determination of amino acids in food products". The amino acid composition of the studied samples was determined according to the M method-04-94-2021 "Determination of amino acids in food products" on the device "Kapel-105M" (GC "LUMEX"), with an autosampler and an automatically switched polarity.
As a result of the conducted research, it was found that the content of interchangeable and essential amino acids in pork of different quality categories depended on the type of amino acids and the quality category of pork. It should be noted that information about the amino acid composition of pork with quality defects should be taken into account in the production of meat products at processing plants.

Leukocyte indices are ratios that are used to assess the state of the patient's blood and immune system. The use of leukocyte indices in the diagnosis of feline diseases is currently limited, which is mainly due to the lack of norms in wide publication, as well as studies of patterns characteristic of specific groups of diseases, as well as the nature of the course of pathological processes. The purpose of the presented study was to assess the indicators of leukocyte indices in clinically healthy cats. The analysis of morphological parameters of the blood of 11 mongrel clinically healthy cats with the obligatory absence of a stress leukogram was carried out. The number of leukocytes and leukogram in the blood were determined according to generally accepted methods, followed by the calculation of leukocyte indices: Krebs index (KI), leukocyte intoxication index (LII), G.D. Dashtayants nuclear index (NI), leukocyte shift index (LSI), leukocyte index (LI), neutrophilmonocyte ratio index (NMRI), the index of the ratio of lymphocytes to monocytes (IRLM). The obtained data are expected to further expand the diagnostic and prognostic capabilities of cat diseases. In the future, the study is planned to be expanded with the capture and analysis of individual nosological units and breed characteristics, as well as the features of the diet, the ratio to the severity of the condition, the analysis of life expectancy and survival. It should be noted that in order to more accurately determine the diagnostic significance and the possibility of determining forecasts, it is necessary to increase the number of animal samples with an assessment of indicators in dynamics.

The morphological features of the carp intestines in the winter period, resulting from the action of negative stress factors, were studied. Due to the violation of the integrity of the ice cover, the constant change in the water level in the wintering pond, which was used for temporary keeping of fish before its sale, the carp is depleted, inflammatory processes in the gastrointestinal tract, which can lead to the death of marketable fish and economic losses for economy. As a result of the studies, necrotic changes, vacuolization in enterocytes were revealed, infiltration of leukocytes was noted in the lamina propria of the mucous membrane.

Growing young chickens under conditions of intensive keeping technology can lead to a decrease in nonspecific resistance, the manifestation of immunodeficiencies and increased susceptibility of the bird to adverse environmental factors and various opportunistic microorganisms. The presence of mycotoxins in feed is one of the main feed stresses. At the same time, stresses associated with vaccinations are added in the future and, of course, heat stresses play a special role in the summer months.
The purpose of the work is to study the influence of the domestic natural adaptogen from the mineral shungite on productivity indicators and on the composition of the intestinal microbiome in young chickens of egg breed.
The object of research was young laying hens of the High Line cross. To conduct the experiment at a day old, 3 groups of young animals were formed (1 control and 2 experimental - with different concentrations of the mineral shungite). The raw material for the production of a mineral additive from shungite was shungite from the Zazhogino deposit (Republic of Karelia, Medvezhyegorsk region). Fraction 0.2-0.8 mm, humidity up to 10%. Carbon content (C) 25-35%.
The composition of the compound feed formula was developed in order to increase the immune status of chickens, accelerate the development of the internal organs of chickens in the early period and, as a result, increase the safety, uniformity and business yield of pullets.
The intestinal microbiome of young laying hens was determined using high throughput sequencing. It should be noted that among the bacteria inhabiting the gastrointestinal tract, the function of digestion is performed by several phyla, the main part of which are bacteria of the phyla Actinobacteria, Firmicutes, Bacteroidetes and Proteobacteria.
Thus, based on the results of high-throughput sequencing, the corrective effect of the adaptogen from shungite at different concentrations on the composition of the bird's intestinal microflora was established. The positive effect of shungite at a concentration of 1.0 kg/t of feed on the increase in the number of normoflora of blind processes was confirmed. A significant effect of the additive from the mineral shungite on the number of cellulolytic bacteria was revealed. 

The work is devoted to the study of the adaptive features of the histological structure of the liver of some representatives of the hare order. The material for the study was organocomplexes from clinically healthy productive rabbits, decorative rabbits and white hare. Research work was carried out at the Department of Anatomy and Physiology of the Federal State Budgetary Educational Institution of Higher Education of the State Agrarian University of the Northern Trans-Urals. Conducted morphometric studies and selected material for morphological and histological studies. The liver of the studied species of the order Lagomorphs has a convex parietal and slightly concave visceral surface. The right and left lobes of the liver are divided into lateral and medial parts. In a productive rabbit and a decorative rabbit, the lateral right lobe is shorter than the lateral left lobe, and in the white hare, both of these are short. The square share of all is an elongated quadrangular shape. Caudate and mastoid processes are small. The gallbladder lies between the right lateral and right medial lobes and does not extend beyond the ventral edge of the liver. Histological studies were carried out according to generally accepted methods. In all species of the order of lagomorphs, formed triads were observed located on the border between the lobules. Hepatocytes form hepatic laminae. Trabeculae and loose fibrous connective tissue are not expressed. Analyzing the obtained histological data, we can say that the adaptive features will include: in rabbits bred under industrial conditions - an increased area of hepatocytes (376.29±22.95 µm2) and nuclei (52.44±4.51 µm2), a decrease in NCR 0.16±0.007; in ornamental rabbits - a decrease in the area of hepatocytes (38.32±1.77 µm2) and nuclei (7.06±0.59 µm2), but the average value of the Nuclear-Cytoplasmic Ratio is 0.42±0.01; in the hare - the average values of the area of hepatocytes (260.79±15.88 µm2) and nuclei (41.58±1.32 µm2), NCR 0.4±0.01. These features indicate significant differences in the nutrition and maintenance of these animals, as well as various adaptation processes.

The venous system of animals always arouses a certain interest among veterinary specialists. On the one hand, there are issues related to comparative anatomy in different animal species, and on the other hand, there is a practical interest put forward by veterinary surgeons and clinicians. First of all, it is certainly the valve apparatus of this system, which gives reason to believe that the valves of the veins not only direct the movement of blood through the venous vessels, but also contribute to balancing the blood pressure in the entire cardiovascular system. The purpose of our study is to study the skeletotopy of the caudal vena cava of Yorkshire piglets, to give anatomical and topographic characteristics of veins, to carry out morphometry of main collectors. To study the branching of the caudal vena cava of Yorkshire piglets, ten corpses aged 28-30 days of life were obtained, which were delivered from the pig breeding complex to the Department of Animal Anatomy of the St. Petersburg State University of Veterinary Medicine. During the study, such methods as: fine anatomical dissection, vasorentgenography, morphometry, photographing were used. As a result of our study, skeletotopy of the caudal vena cava of Yorkshire piglets with anatomical and topographic characteristics of veins was established and morphometry of the main collectors was performed. The topography of the iliac veins in Yorkshire piglets has certain patterns associated with the conditions of maintenance and movement. No valves were found in the common iliac vein, which ensures the outflow of blood from the parietal and visceral branches. A large number of anastomoses were established between the circumferential deep iliac, gluteal and internal sacral veins.

Cytolysis syndrome is a clinical and laboratory complex characterized by an increase in the levels of intracellular biocatalysts of blood transaminases in response to the action of damaging factors, accompanied by their defect and fatal. Cytolysis syndrome can be triggered by infectious and parasitic diseases, metabolic disorders, as well as drug lesions due to polypragmasia. The study of new and effective methods of correction of cytolytic syndrome is an urgent task of clinicians. Vitaminamino acid complex (VAC) "Vitam", due to the presence of a balanced amount of vitamins, amino acids, trace elements and glucose, compensates for the deficiency of these biologically active substances in the body of animals that occurs during hepatosis. The article presents data on the study of the effectiveness of the drug "Vitam" in 45 cats with established cytolytic liver syndrome. The dynamics of biochemical changes in the blood serum of cats with daily administration of the drug for ten days is shown. There was a decrease in the activity of AlAT, AsAT, GGT and LDH, whose indicators reached normal after the course of treatment. The expediency of using the drug "Vitam" in cats with cytolytic syndrome and with subclinical manifestation as therapeutic and metabolic therapy has been established.